The present study was carried out to characterize Salmonella serovars from commercial pig farms in Benin City, Nigeria. A total of 81 samples were collected from the Agricultural Development Program farms between January and June 2017. Standard culture-based and polymerase chain reaction procedures were adopted in the isolation and identification of Salmonella serovars. Antibiotic susceptibility profiles of the isolates were conducted using the Kirby–Bauer disc diffusion method with prominent resistance determinants screened for using specific primer sets. The 84 identified Salmonella serovars include 15 Salmonella ser. Enteritidis, 11 Salmonella ser. Typhimurium, and 58 other Salmonella serovars. Phenotypic virulence factors include: hemolytic activity (51.7–100%), lipase activity (48.3–81.8%), protease activity (60.3–100%), gelatinase production (50–90.9%), DNA degrading activity (55.2–90.9%), and S-layer formation (63.8–100%). The biofilm formation profiles include nonbiofilm producers (0–12.1%), weak biofilm producers (0–20%), moderate biofilm producers (24.1–27.3%), and strong biofilm producers (48.3–72.7%). Salmonella serovars in this study harbored resistant determinants, such as tetA, tetC, ampC, sul1, sul2, sul3, floR, ermA, and ermC. The occurrence of resistance phenotype and determinants in pathogenic Salmonella serovars from pig farms is a significant public health concern, which could result in the dissemination of resistant elements within the environment.

中文翻译：

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从尼日利亚埃多州贝宁市养猪场分离的沙门氏菌血清型的鉴定和表征：一种健康观点

本研究旨在表征来自尼日利亚贝宁市商业养猪场的沙门氏菌血清型。2017 年 1 月至 6 月期间，共从农业发展计划农场收集了 81 个样本。在分离和鉴定沙门氏菌血清型时采用了基于标准的培养和聚合酶链反应程序。使用 Kirby-Bauer 圆盘扩散法对分离株的抗生素敏感性进行了分析，并使用特定引物组筛选了显着的耐药性决定因素。84 种已鉴定的沙门氏菌血清型包括 15 种沙门氏菌系列。肠炎，11沙门氏菌系列。鼠伤寒杆菌和其他 58 种沙门氏菌血清型。表型毒力因子包括：溶血活性（51.7-100%）、脂肪酶活性（48.3-81.8%）、蛋白酶活性（60.3-100%）、明胶酶产生（50-90.9%）、DNA降解活性（55.2-90.9%） , 和 S 层形成 (63.8–100%)。生物膜形成概况包括非生物膜生产者 (0–12.1%)、弱生物膜生产者 (0–20%)、中等生物膜生产者 (24.1–27.3%) 和强生物膜生产者 (48.3–72.7%)。本研究中的沙门氏菌血清型含有耐药决定簇，例如tet A、tet C、amp C、sul 1、sul 2、sul 3、flo R、erm A 和ermC.来自猪场的致病性沙门氏菌血清型中耐药表型和决定因素的出现是一个重大的公共卫生问题，这可能导致耐药元素在环境中的传播。