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Expanding Toolbox for Genes Expression of Yarrowia lipolytica to Include Novel Inducible, Repressible, and Hybrid Promoters.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2020-06-25 , DOI: 10.1021/acssynbio.0c00243 Xiaochao Xiong 1 , Shulin Chen 1
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2020-06-25 , DOI: 10.1021/acssynbio.0c00243 Xiaochao Xiong 1 , Shulin Chen 1
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Promoters are critical tools to precisely control gene expression for both synthetic biology and metabolic engineering. Although Yarrowia lipolytica has demonstrated many industrially relevant advantages, promoter discovery efforts on this non-conventional yeast are limited due to the challenge in finding suitable inducible and repressible promoters. Six copper-inducible promoters and five repressible promoters were isolated in this work. Especially, Cu2+-repressible promoters showed relatively high activity under non-repressing conditions compared with a constitutive promoter, but the strength could be almost fully repressed by a supplement of a low content of Cu2+. The six Cu2+-inducible promoters were engineered to improve their dynamic regulation range with a tandem upstream activation sequence. An engineered promoter was successfully used to construct a more productive pathway for production of a novel bioproduct, wax ester, than that used for both Cu2+-inducible promoter and constitutive promoter. This study provides effective tools applicable to fine-tune the gene expression in this microbial host.
中文翻译：
扩展解脂耶氏酵母基因表达的工具箱，以包括新型诱导型，阻遏型和杂交启动子。
启动子是为合成生物学和代谢工程精确控制基因表达的关键工具。尽管解脂耶氏酵母已显示出许多工业上的相关优势，但由于寻找合适的诱导型和阻遏型启动子所面临的挑战，对该非常规酵母的启动子发现工作受到了限制。这项工作中分离出六个铜诱导型启动子和五个抑制型启动子。特别地，与组成型启动子相比，Cu 2+可抑制型启动子在非抑制条件下显示出相对较高的活性，但是通过添加低含量的Cu 2+可以几乎完全抑制强度。六个Cu 2+诱导型启动子经工程改造以通过串联上游激活序列改善其动态调节范围。与Cu 2+诱导型启动子和组成型启动子相比，工程启动子已成功用于构建新型生物产物蜡酯的生产途径。这项研究提供了可用于微调该微生物宿主中基因表达的有效工具。
更新日期：2020-08-21
中文翻译：
扩展解脂耶氏酵母基因表达的工具箱，以包括新型诱导型，阻遏型和杂交启动子。
启动子是为合成生物学和代谢工程精确控制基因表达的关键工具。尽管解脂耶氏酵母已显示出许多工业上的相关优势，但由于寻找合适的诱导型和阻遏型启动子所面临的挑战，对该非常规酵母的启动子发现工作受到了限制。这项工作中分离出六个铜诱导型启动子和五个抑制型启动子。特别地，与组成型启动子相比，Cu 2+可抑制型启动子在非抑制条件下显示出相对较高的活性，但是通过添加低含量的Cu 2+可以几乎完全抑制强度。六个Cu 2+诱导型启动子经工程改造以通过串联上游激活序列改善其动态调节范围。与Cu 2+诱导型启动子和组成型启动子相比，工程启动子已成功用于构建新型生物产物蜡酯的生产途径。这项研究提供了可用于微调该微生物宿主中基因表达的有效工具。
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