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Carbapenem inactivation method using bacterial lysate and MOPS (LCIM): a very sensitive method for detecting carbapenemase-producing Acinetobacter species.
Journal of Antimicrobial Chemotherapy ( IF 3.9 ) Pub Date : 2020-06-25 , DOI: 10.1093/jac/dkaa238
Kageto Yamada 1, 2 , Kotaro Aoki 2 , Tatsuya Nagasawa 2 , Waka Imai 1 , Masakazu Sasaki 1, 2 , Hinako Murakami 1 , Toshisuke Morita 3 , Yoshikazu Ishii 2 , Kazuhiro Tateda 2
Affiliation  

Detection of carbapenem-hydrolysing class D β-lactamase (CHDL)-producing Acinetobacter spp. is critical for understanding antibiotic resistance. In this study, we compared the available detection techniques derived from the carbapenem inactivation method (CIM), using CHDL-producing Acinetobacter spp., and developed a modified method that uses bacterial lysate (lysate CIM; LCIM).

中文翻译:

使用细菌裂解物和MOPS(LCIM)进行碳青霉烯灭活的方法:一种非常灵敏的方法,用于检测产生碳青霉烯酶的不动杆菌种。

检测产生碳青霉烯的水解D类β-内酰胺酶(CHDL)的不动杆菌属。对于了解抗生素耐药性至关重要。在这项研究中,我们比较了使用产生CHDL的不动杆菌属衍生的碳青霉烯灭活方法(CIM)衍生的可用检测技术,并开发了一种使用细菌裂解物(裂解物CIM; LCIM)的改良方法。
更新日期:2020-06-25
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