Abstract

Alternative splicing (AS) is an important post-transcriptional process to enhance proteome diversity in eukaryotic organisms. In plants, numerous reports have primarily focused on AS analysis in model plant species or herbaceous plants, leading to a notable lack of research on AS in woody plants. More importantly, emerging evidence indicates that many important traits, including wood formation and stress resistance, in woody plants are controlled by AS. In this review article, we summarize the current progress of all kinds of AS studies in different tree species at various stages of development and in response to various stresses, revealing the significant role played by AS in woody plants, as well as the similar properties and differential regulation within their herbaceous counterparts. Furthermore, we propose several potential strategies to facilitate the functional characterization of splicing factors in woody plants and evaluate a general pipeline for the systematic characterization of splicing isoforms in a complex AS regulatory network. The utilization of genetic studies and high-throughput omics integration approaches to analyze AS genes and splicing factors is likely to further advance our understanding of AS modulation in woody plants.

中文翻译：

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选择性剪接及其在木本植物中的调控作用。

摘要

选择性剪接（AS）是增强真核生物蛋白质组多样性的重要转录后过程。在植物中，许多报告主要集中在模型植物物种或草本植物中的AS分析，导致对木本植物中AS的研究明显缺乏。更重要的是，新出现的证据表明，木本植物中的许多重要特征，包括木材形成和抗逆性，均受AS控制。在这篇综述文章中，我们总结了不同树种在发育的各个阶段以及对各种胁迫的响应下各种AS研究的最新进展，揭示了AS在木本植物中的重要作用以及相似的特性和在其草本对应物中的差异调节。此外，我们提出了几种潜在的策略来促进木本植物中剪接因子的功能表征，并评估用于复杂AS监管网络中的剪接异构体的系统表征的一般流程。利用遗传学研究和高通量组学整合方法来分析AS基因和剪接因子，可能会进一步增进我们对木本植物中AS调控的理解。