Noninvasive evaluation of the spatial distribution of chemical composition and diffusion behavior of materials is becoming possible by advanced nuclear magnetic resonance (NMR) pulse sequence editing. However, there is room for improvement in the spectral resolution and analytical method for application to heterogeneous samples. Here, we develop applications for comprehensively evaluating compounds and their dynamics in intact bodies and heterogeneous systems from NMR data, including spatial z-position, chemical shift, and diffusion or relaxation. This experiment is collectively named spatial molecular-dynamically ordered spectroscopy (SMOOSY). Pseudo-three-dimensional (3D) SMOOSY spectra of an intact shrimp and two heterogeneous systems are recorded to evaluate this methodology. Information about dynamics is mapped onto two-dimensional (2D) chemical shift imaging spectra using a pseudo-spectral imaging method with a processing tool named SMOOSY processor. Pseudo-2D SMOOSY spectral images can non-invasively assess the different dynamics of the compounds at each spatial z-position of the shrimp’s body and two heterogeneous systems.

通过先进的核磁共振 (NMR) 脉冲序列编辑，对材料的化学成分和扩散行为的空间分布进行无创评估成为可能。然而，在应用于异质样品的光谱分辨率和分析方法方面仍有改进的空间。在这里，我们开发了用于根据 NMR 数据（包括空间z）综合评估化合物及其在完整体和异质系统中的动力学的应用-位置、化学位移和扩散或弛豫。该实验统称为空间分子动力学有序光谱学（SMOOSY）。记录了完整虾和两个异构系统的伪三维 (3D) SMOOSY 光谱以评估该方法。使用伪光谱成像方法和名为 SMOOSY 处理器的处理工具将有关动力学的信息映射到二维 (2D) 化学位移成像光谱。伪二维 SMOOSY 光谱图像可以非侵入性地评估化合物在虾体和两个异质系统的每个空间z位置的不同动力学。