Autologous conditioned serum (ACS) and autologous protein solution (APS) are newer therapeutic options for osteoarthritis (OA). Co-culture of cartilage and synovium stimulated with IL-1β produces a similar physiologic response to tissues from naturally-ocurring OA. The study objective was to investigate the effects of ACS, APS, and triamcinolone (TA) on inflammatory and catabolic gene expression of inflamed joint tissues in co-culture. Blood was collected and processed for ACS and APS from six horses. Cartilage and synovial explants were harvested from the stifle, placed in co-culture, and treated as: (1) unstimulated control (2) stimulated control (3) ACS at 25% v/v (4) ACS at 50% v/v (5) APS at 25% v/v (6) APS at 50% v/v, (7) TA (10⁻⁶ M). Treatment groups 2–7 were stimulated with IL-1β (10 ng/ml). Cultures were maintained for 96 hours, and then both media and explants were harvested for measurement of gene expression and protein. IL-1β stimulation significantly increased IL-1β (p = 0.029), IL-8 (p = 0.011) and MMP-3 (p = 0.043) expression in synovium and IL-1β (p = 0.003) and TNF-α (p = 0.001) expression in cartilage. Treatment with 50% ACS and APS v/v downregulated IL-1β expression in cartilage more than TA treatment (p = 0.001 and p = 0.0004) and APS downregulated MMP-1 expression in synovial membrane (p = 0.025). Treatment with ACS and APS caused a trend in upregulation of IL-10 expression in synovium and type II collagen and aggrecan expression in cartilage. PGE₂ media concentrations were significantly reduced following treatment with APS (13.7-fold decrease, p = 0.0001) and ACS (4.13-fold decrease, p = 0.024); while TA did not reduce PGE₂ significantly (2.3-fold decreased p = 0.406). As disease-modifying therapies, ACS and APS modified the cellular response from synovial membrane and articular cartilage. ACS and APS may offer an improved strategy to improve clinical signs of horses with naturally occurring OA, compared to TA treatment.

自体条件血清（ACS）和自体蛋白溶液（APS）是骨关节炎（OA）的较新治疗选择。用IL-1β刺激的软骨和滑膜的共培养对天然OA产生的组织产生相似的生理反应。研究目的是研究ACS，APS和曲安西龙（TA）对共培养中炎症关节组织的炎症和分解代谢基因表达的影响。从六匹马中收集血液并进行ACS和APS处理。从窒息物中收获软骨和滑膜外植体，放置于共培养物中，并按以下方式处理：（1）未刺激的对照（2）受刺激的对照（3）25％v / v的ACS（4）50％v / v的ACS （5）25％v / v时的APS（6）50％v / v时的APS，（7）TA（10 ^-6M）。IL-1β（10 ng / ml）刺激治疗组2-7。维持培养96小时，然后收获培养基和外植体用于测量基因表达和蛋白质。IL-1β刺激显着增加IL-1β（p = 0.029），IL-8（p = 0.011）和MMP-3（p = 0.043）在滑膜和IL-1β（p = 0.003）和TNF-α（p= 0.001）在软骨中的表达。用50％ACS和APS v / v进行治疗的软骨中IL-1β表达下调的程度比TA治疗要高（p = 0.001并且 p = 0.0004）和APS下调滑膜中MMP-1的表达（p= 0.025）。ACS和APS的治疗引起滑膜和II型胶原中IL-10表达上调以及软骨中聚集蛋白聚糖表达上调的趋势。APS处理后，PGE ₂培养基浓度显着降低（降低了13.7倍，p = 0.0001）和ACS（降低4.13倍， p= 0.024）；而TA并未显着降低PGE ₂（降低了2.3倍p= 0.406）。作为改变疾病的疗法，ACS和APS改变了滑膜和关节软骨的细胞反应。与TA治疗相比，ACS和APS可能会提供一种改善策略，以改善患有天然OA的马的临床体征。