Zophobas morio is a tropical darkling beetle which is widely exploited for commercial large-scale insect growing. Outbreaks of a disease may occur causing total devastation of cultures. In the present paper, samples of diseased Z. morio were obtained and used for establishment of a laboratory model as they were found infective to the larvae of the same insect species from another source. It took about 1 month to develop symptoms of acute disease in mid-age larvae and about twice as much when younger larvae were used for infection. Affected larvae perished quickly, and within several days up to 90–100% of the colony could perish. Both in healthy and diseased larvae a virus was detected using PCR with degenerate primers specific for a gene coding for a non-structural protein (ORF3). The sequenced gene fragment (Genbank accession #MN732869) confirmed allocation of the virus to Densoviridae, with maximal similarity of 97.2% to Blatella germanica densovirus-like virus (#JQ320376) and 66.2% to B. germanica densovirus (#AY189948). Genomic DNA samples of Z. morio larvae from an independent colony devoid of symptoms of a disease were also positive for this virus with a slightly different (99.7% sequence similarity to the former sequence of the Z. morio densovirus) genotype (#MN732870).
Intervirology

中文翻译：

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在健康和患病的Zophobas morio（鞘翅目，Tenebrionidae）中的登革病毒的分子鉴定。

Zophobas morio是一种热带黑夜甲虫，被广泛用于商业化大规模昆虫生长。疾病爆发可能会引起文化的全面破坏。在本文中，患病的Z. morio的样本由于发现它们感染了另一种来源的相同昆虫物种的幼虫，因此获得了它们并用于建立实验室模型。在中年幼虫中出现急性疾病的症状大约需要1个月，而使用幼虫进行感染大约要花费两倍的时间。受影响的幼虫很快灭绝，几天之内最多可灭绝90-100％的菌落。在健康幼虫和患病幼虫中，均使用PCR检出了一种病毒，该病毒使用了对非结构蛋白（ORF3）编码基因具有特异性的简并引物。测序的基因片段（Genbank登录号为MN732869）证实了该病毒已分配给Densoviridae，与德国小den齿状病毒样病毒（＃JQ320376）的最大相似性为97.2％，与德国双歧杆菌的最大相似性为66.2％。densovirus（＃AY189948）。来自没有疾病症状的独立菌落的Z. morio幼虫的基因组DNA样品对该病毒也呈阳性，其基因型（＃MN732870）的基因型略有不同（与Z. morio densovirus的先前序列有99.7％的序列相似性）。
病毒学