Infrared attenuated total reflection spectroscopic surface analysis of bovine-tail intervertebral discs after UV-light-activated riboflavin-induced collagen cross-linking.,Journal of Biophotonics

当前位置： X-MOL 学术 › J. Biophotonics › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Infrared attenuated total reflection spectroscopic surface analysis of bovine-tail intervertebral discs after UV-light-activated riboflavin-induced collagen cross-linking.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-06-26 , DOI: 10.1002/jbio.202000110
Ioannis Vasilikos ₁ , Julian Haas ₂ , Graciosa Q Teixeira ₃ , Julia Nothelfer ₃ , Cornelia Neidlinger-Wilke ₃ , Hans-Joachim Wilke ₃ , Andreas Seitz ₃ , Demetrios G Vavvas ₄ , Josef Zentner ₁ , Jürgen Beck ₁ , Ulrich Hubbe ₁ , Boris Mizaikoff ₂

Affiliation

The tensile strength of the intervertebral disc (IVD) is mainly maintained by collagen cross‐links. Loss of collagen cross‐linking combined with other age‐related degenerative processes contributes to tissue weakening, biomechanical failure, disc herniation and pain. Exogenous collagen cross‐linking has been identified as an effective therapeutic approach for restoring IVD tensile strength. The current state‐of‐the‐art method to assess the extent of collagen cross‐linking in tissues requires destructive procedures and high‐performance liquid chromatography. In this study, we investigated the utility of infrared attenuated total reflection (IR‐ATR) spectroscopy as a nondestructive analytical strategy to rapidly evaluate the extent of UV‐light‐activated riboflavin (B2)‐induced collagen cross‐linking in bovine IVD samples. Thirty‐five fresh bovine‐tail IVD samples were equally divided into five treatment groups: (a) untreated, (b) cell culture medium Dulbecco's Modified Eagle's Medium only, (c) B2 only, (d) UV‐light only and (e) UV‐light‐B2. A total of 674 measurements have been acquired, and were analyzed via partial least squares discriminant analysis. This classification scheme unambiguously identified individual classes with a sensitivity >91% and specificity >92%. The obtained results demonstrate that IR‐ATR spectroscopy reliably differentiates between different treatment categories, and promises an excellent tool for potential in vivo, nondestructive and real‐time assessment of exogenous IVD cross‐linking.

中文翻译：

紫外光活化核黄素诱导的胶原交联后，牛尾椎间盘的红外衰减全反射光谱表面分析。

椎间盘（IVD）的拉伸强度主要通过胶原蛋白交联来维持。胶原蛋白交联的丧失与其他与年龄有关的退化过程相结合，导致组织变弱，生物力学衰竭，椎间盘突出和疼痛。外源性胶原交联被认为是恢复IVD拉伸强度的有效治疗方法。当前评估组织中胶原蛋白交联程度的最先进方法需要破坏性程序和高效液相色谱法。在这项研究中，我们调查了红外衰减全反射（IR‐ATR）光谱作为一种无损分析策略的实用性，以快速评估牛IVD样品中紫外线激活的核黄素（B2）诱导的胶原蛋白交联的程度。将35份新鲜牛尾IVD样品平均分为五个治疗组：（a）未经处理的（b）仅细胞培养基Dulbecco's Modified Eagle's培养基，（c）仅B2，（d）仅UV-光和（e））紫外线B2。总共获得了674个测量值，并通过偏最小二乘判别分析进行了分析。该分类方案明确地确定了灵敏度> 91％和特异性> 92％的单个类别。获得的结果表明，IR-ATR光谱学能够可靠地区分不同的治疗类别，并有望成为潜在的体内，无损和实时评估外源IVD交联的极佳工具。s仅中等，（c）仅B2，（d）仅紫外光和（e）紫外光B2。总共获得了674个测量值，并通过偏最小二乘判别分析进行了分析。该分类方案明确地确定了灵敏度> 91％和特异性> 92％的单个类别。获得的结果表明，IR-ATR光谱学能够可靠地区分不同的治疗类别，并有望成为潜在的体内，无损和实时评估外源IVD交联的极佳工具。s仅中等，（c）仅B2，（d）仅紫外光和（e）紫外光B2。总共获得了674个测量值，并通过偏最小二乘判别分析进行了分析。该分类方案明确地确定了灵敏度> 91％和特异性> 92％的单个类别。所得结果表明，IR-ATR光谱学能够可靠地区分不同的治疗类别，并有望成为潜在的体内，无损和实时评估外源IVD交联的极佳工具。

更新日期：2020-06-26

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11