Poly(N-isopropylacrylamide)-Based Polymers as Additive for Rapid Generation of Spheroid via Hanging Drop Method.,Macromolecular Bioscience

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Poly(N-isopropylacrylamide)-Based Polymers as Additive for Rapid Generation of Spheroid via Hanging Drop Method.
Macromolecular Bioscience ( IF 4.4 ) Pub Date : 2020-06-26 , DOI: 10.1002/mabi.202000180
Vignesh R A ₁ , Shalini Kumari ₁ , Puja Poddar ₂ , Dibakar Dhara ₂ , Souvik Maiti _{1,

3}

Affiliation

Multicellular tumor spheroid (MCTS) mimics microenvironment for tumor formation and provides predictive insight for in vivo tests. The hanging drop (HD) method of spheroid generation is cost effective, but it is limited by a long time duration for spheroid development and a low rate of formation of larger spheroids. Toward addressing those limitations, thermoresponsive copolymers with poly(N‐isopropylacrylamide) (p(NIPA)) backbone are developed, to be used as additives in the MCTS formation via HD method. Upon investigation it is found that in the presence of the polymer, robust and compact spheroids are formed in a short duration of 48 h. Larger spheroids (350–600 µm) can be formed by increasing the number of cells. Spheroids are characterized for their 3D shape and different cellular layers, and drug uptake study is done to prove the efficacy of the spheroids generated in drug screening.

中文翻译：

聚（N-异丙基丙烯酰胺）基聚合物作为通过悬滴法快速生成球体的添加剂。

多细胞肿瘤球体（MCTS）模仿了肿瘤形成的微环境，并为体内测试提供了预测性见解。球状体的悬滴法（HD）具有成本效益，但受到球状体发育时间长和较大球状体形成率低的限制。为了解决这些局限性，聚（N开发了异丙基丙烯酰胺（p（NIPA））骨架，通过HD方法将其用作MCTS形成中的添加剂。经研究发现，在聚合物存在下，在48小时的短时间内就形成了坚固而致密的球体。通过增加细胞数量可以形成较大的球状体（350–600 µm）。球体的特征在于其3D形状和不同的细胞层，并且进行了药物吸收研究以证明在药物筛选中产生的球体的功效。

更新日期：2020-06-26

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