Bone tissue engineering is gaining popularity as an alternative method for the treatment of osseous defects. A number of biodegradable polymers have been explored for tissue engineering purposes. A new family of biodegradable polymer/bioactive glass composite materials has been designed to be used in bone regeneration approaches. In this work, a hybrid scaffold of chitosan (CH) and bioactive glass nanoparticles (BGN) was prepared by the freeze‐gelation method. This method has been studied by adjusting the concentration of acetic acid; this process can influence the structure properties of the scaffold. In this work, several BGN/CH composites have been prepared by varying the proportion of BGN in the hybrid scaffold (20, 40, 60, and 80%). Brunauer–Emmett–Teller results showed the increased surface area and porosity volume of our composite with decreasing BGN proportion. BGN/CH hybrid scaffold was characterized by using physicochemical techniques. Obtained results showed a macroporous morphology of the scaffold with a pore size of about 200 μm, and a homogeneous distribution of the BGN in the CH matrix. X‐ray diffraction study confirmed the amorphous state of the BGN/CH hybrid scaffold. Interaction between CH and BGNs in the composite was confirmed. The in vitro assays showed adequate degradation properties, which is essential for the potential replacement by the new tissue. The in vitro bioactivity studies confirmed the formation of an apatite layer on the surface of the hybrid scaffold, which results in a direct bone bonding of the implant. These results indicate that BGN/CH hybrid scaffold developed is a potential candidate for bone tissue engineering.

中文翻译：

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混合支架的开发：用于组织工程应用的生物活性玻璃纳米粒子/壳聚糖

骨组织工程作为治疗骨缺损的替代方法越来越受欢迎。许多可生物降解的聚合物已被探索用于组织工程目的。一种新的可生物降解聚合物/生物活性玻璃复合材料系列已被设计用于骨再生方法。在这项工作中，通过冷冻凝胶法制备了壳聚糖（CH）和生物活性玻璃纳米粒子（BGN）的混合支架。该方法通过调节乙酸浓度进行了研究；这个过程会影响支架的结构特性。在这项工作中，通过改变混合支架中 BGN 的比例（20%、40%、60% 和 80%）制备了几种 BGN/CH 复合材料。Brunauer-Emmett-Teller 结果表明，我们的复合材料的表面积和孔隙率随着 BGN 比例的降低而增加。BGN/CH 混合支架通过使用物理化学技术进行表征。获得的结果显示支架具有大孔形态，孔径约为 200 μm，BGN 在 CH 基质中均匀分布。X 射线衍射研究证实了 BGN/CH 杂化支架的无定形状态。确认了复合材料中 CH 和 BGN 之间的相互作用。体外测定显示出足够的降解特性，这对于新组织的潜在替代至关重要。体外生物活性研究证实了在混合支架表面形成磷灰石层，从而导致植入物直接骨结合。