Palindromic peptide LfcinB (21–25)_Pal: RWQWRWQWR was synthesized by Solid Phase Peptide Synthesis (SPPS‐Fmoc/t Bu), purified by Reverse Phase Solid Phase Extraction (RP‐SPE) and characterized by Reverse Phase High Performance Liquid Chromatography (RP‐HPLC) and Matrix‐Assisted Laser Desorption/Ionization‐Time Of Flight Mass Spectrometry (MALDI‐TOF MS). The antifungal activity of LfcinB (21–25)_Pal against both ATCC strains and clinical isolates of C. albicans, C. glabrata, C. krusei, C. auris and C. tropicalis was evaluated. The palindromic peptide exhibited fungistatic and fungicidal activity against all yeast evaluated. The antifungal activity was dependent on peptide concentration in all cases. Additionally, LfcinB (21–25)_Pal (25–50 μg/mL) combined with fluconazole exhibited a synergistic antifungal effect against C. tropicalis 883 and C. krusei 6258 (resistant to fluconazole). This study showed that the palindromic peptide derived from Lactoferricin B (LfcinB) exhibited significant antifungal activity against Candida spp, suggesting that this peptide could have a therapeutic application solely or in combination with fluconazole.

中文翻译：

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回文肽LfcinB（21-25）Pal对多药耐药念珠菌具有抗真菌活性

回文肽LfcinB（21–25）_Pal：RWQWRWQWR通过固相肽合成（SPPS-Fmoc / t Bu）合成，通过反相固相萃取（RP-SPE）纯化，并通过反相高效液相色谱（RP）进行表征HPLC）和基​​质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）。LfcinB（21–25）_Pal对ATCC菌株和白色念珠菌，光滑念珠菌，克鲁斯酵母，金黄色葡萄球菌和热带念珠菌的临床分离株均具有抗真菌活性。被评估。回文肽对评估的所有酵母菌均显示出抑菌和杀真菌活性。在所有情况下，抗真菌活性均取决于肽浓度。此外，LfcinB（21–25）_Pal（25–50μg/ mL）与氟康唑联用，对热带假丝酵母883和克鲁斯酵母6258（对氟康唑有抗性）表现出协同的抗真菌作用。这项研究表明，源自乳铁蛋白B的回文肽（LfcinB）对念珠菌具有显着的抗真菌活性，表明该肽可以单独或与氟康唑联用。