Quantitative analysis of amino acids in blood and urine is primarily indicated for the diagnosis of amino acid disorders. The high‐performance liquid chromatography (HPLC) technique is frequently used for this detection. The frequency of sample collection on filter paper has been increasing exponentially, and there are many advantages attributed to processing biological samples in this way. The aim of this study was to validate a quantitative analysis of amino acids by HPLC in blood and urine collected on filter paper and to establish reference values in the neonatal period. Dried blood and dried urine samples of respectively 58 and 45 healthy newborns (2–9 days) were collected. Pre‐treatment and extraction of samples were done according to the literature. Separation and analysis of amino acids were carried out by HPLC with fluorescence detection. The developed method demonstrated excellent separation, linearity, limits of detection and quantification, repeatability and recovery. The reference values for 17 amino acids were defined in dried blood and urine samples of newborns. This work presents a simple, fast and effective method for the simultaneous analysis of 17 amino acids in blood and urine collected on filter paper in a single run. The reference values were established and validated.

中文翻译：

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通过HPLC对新生儿期干血和尿液中的氨基酸进行定量分析：建立参考值。

血液和尿液中氨基酸的定量分析主要用于诊断氨基酸疾病。高效液相色谱（HPLC）技术通常用于此检测。在滤纸上收集样品的频率呈指数增长，并且以这种方式处理生物样品具有许多优势。这项研究的目的是验证高效液相色谱法对滤纸上收集的血液和尿液中氨基酸的定量分析，并确定新生儿时期的参考值。分别收集了58和45个健康新生儿（2–9天）的干血和尿样。根据文献进行样品的预处理和提取。氨基酸的分离和分析通过具有荧光检测的HPLC进行。所开发的方法表现出出色的分离度，线性，检测和定量限，可重复性和回收率。在新生儿的干血和尿液样本中定义了17种氨基酸的参考值。这项工作提出了一种简单，快速，有效的方法，可同时分析一次收集在滤纸上的血液和尿液中的17种氨基酸。建立并验证了参考值。