Virus communities of forest fungi remain poorly characterized. In this study, we detected two new viruses co-infecting an isolate of the polypore fungus Bondarzewia berkeleyi using high-throughput sequencing. One of them was a putative new partitivirus designated as Bondarzewia berkeleyi partitivirus 1 (BbPV1), with two linear dsRNA genome segments of 1928 and 1863 bp encoding a putative RNA-dependent RNA polymerase (RdRP) of 591 aa and a putative capsid protein of 538 aa. The other virus, designated as Bondarzewia berkeleyi negative-strand RNA virus 1 (BbNSRV1), had a non-segmented negative-sense RNA genome of 10,983 nt and was related to members of family Mymonaviridae. The BbNSRV1 genome includes six predicted open reading frames (ORFs) of 279, 425, 230, 174, 200 and 1970 aa. The longest ORF contained conserved regions corresponding to Mononegavirales RdRP and mRNA-capping enzyme region V constituting the mononegavirus Large protein. In addition, a low level of sequence identity was detected between the putative nucleocapsid protein-coding ORF2 of Lentinula edodes negative-strand RNA virus 1 and BbNSRV1. The viruses characterized in this study are the first ones described in Bondarzewia spp., and BbNSRV1 is the second mymona-like virus described in a basidiomycete host.

森林真菌的病毒群落特征仍然很差。在这项研究中，我们使用高通量测序检测了两种新病毒，共同感染了一种多孢真菌Bondarzewia berkeleyi分离株。其中之一是一种被认为是新型的Partitivirus，称为Bondarzewia berkeleyi partitivirus 1（BbPV1），具有两个线性dsRNA基因组片段，分别为1928和1863 bp，编码一个假定的依赖于RNA的RNA聚合酶（RdRP）为591aa，一个假定的衣壳蛋白为538啊 其它病毒，命名为Bondarzewia berkeleyi负链RNA病毒1（BbNSRV1），具有10983个核苷酸的非节段负链RNA基因组，并与家庭成员Mymonaviridae。BbNSRV1基因组包括六个预测的开放阅读框（ORF），分别为279、425、230、174、200和1970氨基酸。最长的ORF包含对应于Mononegavirales RdRP的保守区和构成单负病毒大蛋白的mRNA封端酶区V。此外，在香菇负链RNA病毒1的假定的编码核衣壳蛋白ORF2与BbNSRV1之间检测到低水平的序列同一性。在这项研究中表征的病毒是Bondarzewia spp。中描述的第一种病毒，而BbNSRV1是在担子菌宿主中描述的第二种类真菌病病毒。