Glutathione (GSH) is closely related to many physiological processes, the sensitive determination of GSH is significant to the diagnostic and treatment of the specific diseases. In this work, an activatable bimodal imaging and determination strategy of GSH is proposed based on the nanocomposite of black phosphorus quantum dots (BPQDs) wrapped with manganese dioxide nanosheets (BPQDs@MnO₂). The quenched fluorescence of BPQDs can be recovered with the addition of GSH, as a result of the reduction of MnO₂. The BPQDs@MnO₂ appears a linearly activatable fluorescence to the addition of GSH from 0.1 μM to 60 μM with a limit of detection of 35 nM (3σ/s). As the Mn²⁺ produced by the reduction of MnO₂ is a good magnetic resonance imaging (MRI) contrast agent, the BPQDs@MnO₂ is applied to the fluorescence/MRI bimodal imaging of intracellular GSH. MTT experiments prove the good biocompatibility of BPQDs@MnO₂, and different kinds of cells are used to demonstrated that bimodal imaging intensity of cells incubated with BPQDs@MnO₂ was dependent on the concentration of intracellular GSH. This strategy provided a new method for the activatable determination of GSH, and offered a promising platform for fluorescence/MRI bimodal imaging of GSH in living cells.

谷胱甘肽（GSH）与许多生理过程密切相关，对GSH的敏感测定对特定疾病的诊断和治疗具有重要意义。在这项工作中，基于包裹有二氧化锰纳米片（BPQDs @ MnO ₂）的黑磷量子点（BPQDs）的纳米复合物，提出了一种可激活的GSH双峰成像和测定策略。由于MnO ₂的还原，可以通过添加GSH回收BPQD的猝灭荧光。BPQDs @ MnO ₂在从0.1μM到60μM的GSH加入中表现出线性可激活的荧光，检测限为35 nM（3σ/ s）。作为MnO ₂还原生成的Mn ²⁺BPQDs @ MnO ₂是一种良好的磁共振成像（MRI）造影剂，应用于细胞内GSH的荧光/ MRI双峰成像。MTT实验证明BPQDs @ MnO ₂具有良好的生物相容性，并通过不同种类的细胞来证明BPQDs @ MnO ₂培养的细胞的双峰成像强度取决于细胞内GSH的浓度。该策略为可激活的GSH测定提供了一种新方法，并为活细胞中GSH的荧光/ MRI双峰成像提供了有希望的平台。