Abstract Biotechnology is developed to introduce better services to human life. In this way, the current manuscript focuses on the development of exopolysaccharides (EPSs) produced by Cordyceps militaris to face the problems of low productivity and high downstream separation cost. The experiment of repeated batch fermentation coupling with foam separation in the presence of a surfactant was designed to achieve this goal. The EPSs production reached to 1.313 g/L by adding 0.1% (v/v) of tween 80 at the 4th day. Repeated batch fermentation technology coupling with foam separation was proceeded for five batches in 13 days. During the coupling process, the enrichment ratio and recovery percentage of EPSs were maintained at 2.4 and 60%, respectively. However, at recycling processes, the mycelium from foamate of batch II displayed EPSs production of 1.567 g/L, and it was higher than the control group (1.024 g/L). The study demonstrated that this technology would be useful in scaling-up of the fermentation process for improving the EPSs production.

中文翻译：

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在表面活性剂存在下应用复批发酵技术结合泡沫分离提高真菌EPSs产量

摘要 生物技术的发展是为了为人类生活提供更好的服务。这样，目前的手稿重点是开发蛹虫草生产的胞外多糖（EPSs），以解决生产效率低和下游分离成本高的问题。设计了在表面活性剂存在下重复分批发酵与泡沫分离耦合的实验来实现这一目标。通过在第 4 天添加 0.1% (v/v) 的吐温 80，EPS 产量达到 1.313 g/L。重复分批发酵技术与泡沫分离相结合，在13天内进行了5批次。在偶联过程中，EPSs的富集率和回收率分别保持在2.4%和60%。然而，在回收过程中，第 II 批泡沫中的菌丝体显示 EPSs 产量为 1。567 g/L，高于对照组（1.024 g/L）。该研究表明，该技术将有助于扩大发酵过程以提高 EPS 产量。