In this study, multi-layered nanofiber systems consisting of polycaprolactone/chitosan (PCL/CS) polymers loaded with anticancer drugs, methotrexate (MTX) and 5-fluorouracil (5-FU), were designed and produced. The surface morphology of drug-free and drug-loaded samples was investigated by field-emission scanning electron microscopy (FESEM). Fourier transform infrared spectrometry (FTIR) was employed to study the chemical structure of polymers and drugs. The drug release test in neutral and acidic medium (pH 7.4 and pH 4.4) was carried out, and the released drug concentration was measured using UV-spectrophotometry. Moreover, the mechanical properties of single- and multi-layered samples were also studied. The FESEM images indicated the production of uniform and bead-free fibers. FTIR tests confirmed the presence of the drugs in the polymer mixture. The results revealed higher degradation and weight loss percentage in samples with higher CS content due to the hydrophilic nature of CS. Evaluating the drug release behavior of MTX and 5-FU for 624 h revealed that seven-layered structures have prolonged drug release profile and lower burst release rate. The comparison of the release profile of MTX and 5-FU in neutral and acidic environments showed that the rate and amount of release increase with decreasing the pH value. Also, results indicated that the drug release of nanofibers followed Fickian diffusion mechanism and Higuchi model kinetic.

在这项研究中，设计并生产了由载有抗癌药，甲氨蝶呤（MTX）和5-氟尿嘧啶（5-FU）的聚己内酯/壳聚糖（PCL / CS）聚合物组成的多层纳米纤维系统。通过场发射扫描电子显微镜（FESEM）研究了无药物和载药样品的表面形态。傅里叶变换红外光谱（FTIR）用于研究聚合物和药物的化学结构。在中性和酸性介质（pH 7.4和pH 4.4）中进行药物释放测试，并使用紫外分光光度法测量释放的药物浓度。此外，还研究了单层和多层样品的机械性能。FESEM图像表明产生了均匀且无珠的纤维。FTIR测试证实了药物在聚合物混合物中的存在。结果表明，由于CS的亲水性，具有较高CS含量的样品具有更高的降解率和失重率。对MTX和5-FU的药物释放行为进行了624小时的评估，发现七层结构具有较长的药物释放曲线和较低的爆发释放速率。在中性和酸性环境中MTX和5-FU的释放曲线比较表明，释放速率和释放量随pH值的降低而增加。此外，结果表明，纳米纤维的药物释放遵循Fickian扩散机制和Higuchi模型动力学。对MTX和5-FU的药物释放行为进行了624小时的评估，发现七层结构具有较长的药物释放曲线和较低的爆发释放速率。在中性和酸性环境中MTX和5-FU的释放曲线比较表明，释放速率和释放量随pH值的降低而增加。此外，结果表明，纳米纤维的药物释放遵循Fickian扩散机制和Higuchi模型动力学。对MTX和5-FU的药物释放行为进行了624小时的评估，发现七层结构具有较长的药物释放曲线和较低的爆发释放速率。在中性和酸性环境中MTX和5-FU的释放曲线比较表明，释放速率和释放量随pH值的降低而增加。此外，结果表明，纳米纤维的药物释放遵循Fickian扩散机制和Higuchi模型动力学。