Reproductive fluids from the female reproductive tract are gaining attention for their potential to support and optimize reproductive processes, including gamete maturation and embryo culture in vitro. Quantitative proteomics is a powerful way to decipher the proteome of reproductive tract fluids and to identify biologically relevant proteins. The present review describes proteomic strategies for analysing female reproductive fluid proteins. In addition, it considers the strategies for the preparation of oviductal, uterine and follicular fluid samples. Finally, it highlights the main results of quantitative proteomic studies, providing insights into the biological processes related to reproductive biology in farm animals.

Significance

Assisted reproductive technologies (ART) have become vitally important for farm animal breeding and much effort is going into the optimization and refinement of the techniques. There are also attempts to imitate physiological conditions by adding reproductive fluids or individual fluid proteins to improve in vitro procedures. A detailed knowledge of the reproductive fluid proteomes is indispensable. The present review summarizes the most widely used quantitative proteomic approaches for the analysis of fluids from the female reproductive tract and highlights the potential of quantitative proteomics to delineate reproductive processes and identify candidate proteins for ART in farm animals.

中文翻译：

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研究家畜繁殖的蛋白质组学定量策略：雌性生殖液。

女性生殖道的生殖液因其支持和优化生殖过程（包括配子成熟和体外胚胎培养）的潜力而受到关注。定量蛋白质组学是破译生殖道蛋白质组和鉴定生物学相关蛋白质的有力方法。本综述描述了蛋白质组学策略，用于分析女性生殖液蛋白。此外，它还考虑了制备输卵管，子宫和卵泡液样品的策略。最后，它突出了定量蛋白质组学研究的主要成果，提供了与农场动物生殖生物学相关的生物学过程的见解。

意义

辅助生殖技术（ART）对于农场动物育种已变得至关重要，并且正在对技术进行优化和完善。还尝试通过添加生殖液或单个液体蛋白来模仿生理状况，以改善体外程序。生殖液蛋白质组学的详细知识是必不可少的。本综述总结了用于分析女性生殖道液体的最广泛使用的定量蛋白质组学方法，并强调了定量蛋白质组学勾勒出生殖过程并鉴定用于家畜的ART候选蛋白的潜力。