Three new ruthenium(II)-arene complexes, [Ru(η⁶-p-cymene)(L¹)Cl₂] (C1) where L¹ is N-((4 methoxyphenyl)carbamothioyl)benzamide; [Ru(η⁶-p-cymene)(L²)Cl₂] (C2) where L² is 4-(3-benzoylthioureido)benzoic acid and [Ru(η⁶-p-cymene)(L³)Cl₂] (C3) where L³ is methyl 4-(3- benzoylthioureido)benzoate have been synthetized, characterized and evaluated for their antimicrobial and anticancer activity. Characterization was performed using ¹H and ¹³C NMR, IR spectroscopy, mass spectrometry, electrical conductivity measurements and X-Ray diffraction analysis. X-Ray diffraction analysis of C1 showed typical expected “piano-stool” geometry with ruthenium coordinated to ligand via nitrogen and sulfur atoms of benzoylthiourea derivatives. Interesting, in herein described complex, upon coordination the four-membered ring was formed, instead of six-membered chelate common for this type of ligands. Cytotoxic activity was determined in human cervix adenocarcinoma (HeLa) cell line and IC₅₀ values ranged from 29.68 to 52.36 μM and the complexes were more active than related ligands (except in case of C2 where it is found that IC₅₀ value is close to IC₅₀ value of related ligand). Complex [Ru(η⁶-p-cymene)(L¹)Cl₂] (C1) expressed the highest cytotoxic activity with IC₅₀ value of 29.7 μM. Complexes and ligands were tested against nine Gram-positive and Gram-negative bacteria and one yeast- Candida albicans. Clinical Candida spp. strains from microbiological laboratories were included in testing processes as well. Minimum inhibitory concentrations values ranged from 62.5 μg/ml for complexes against Candida albicans to over 1000 μg/ml for several bacterial species.

三个新的钌（II）配合物-arene，的[Ru（η ⁶ -对异丙基苯甲烷）（L ¹）氯₂ ] （C1） ，其中L ¹是N - （（4-甲氧基苯基）硫代氨基甲酰基）苯甲酰胺; 的[Ru（η ⁶ -对异丙基苯甲烷）（L ²）氯₂ ] （C2） ，其中L ²是4-（3- benzoylthioureido）苯甲酸和的[Ru（η ⁶ -对异丙基苯甲烷）（L ³）氯₂ ] （C3） ，其中L ³是甲基-4-（3- benzoylthioureido）苯甲酸甲酯已经synthetized，其特征在于，评价它们的抗菌和抗癌活性。使用¹进行表征H和¹³ C NMR，IR光谱，质谱，电导率测量和X射线衍射分析。C1的X射线衍射分析显示出典型的预期“钢琴凳”几何形状，其中钌通过苯甲酰基硫脲衍生物的氮和硫原子与配体配位。有趣的是，在本文描述的复合物中，在配位时形成四元环，而不是这种配体常见的六元螯合物。在人宫颈腺癌细胞（HeLa）细胞系中测定了细胞毒活性，IC ₅₀值在29.68至52.36μM之间，并且该复合物比相关配体更具活性（发现C2的情况下，IC ₅₀值接近于IC ₅₀相关配体的值）。复杂的[Ru（η ⁶ -对异丙基苯甲烷）（L ¹）氯₂ ] （C1）表示与IC最高的细胞毒活性₅₀ 29.7μM的值。测试了9种革兰氏阳性和革兰氏阴性细菌以及1种酵母白色念珠菌的复合物和配体。临床念珠菌属。来自微生物实验室的菌株也包括在测试过程中。最小抑制浓度值范围从针对白色念珠菌的复合物的62.5μg/ ml到几种细菌物种的1000μg/ ml以上。