The objective of this study was to screen Lactobacillus plantarum strains isolated from the traditional Slovak raw sheep milk cheese for their inhibitory potential. Seventy-two strains were obtained from samples of raw sheep milk and raw sheep milk cheeses collected from April 2017 to September 2018, in 23 geographical areas of Eastern Slovakia, by inoculation of de Man, Rogosa, and Sharpe agar plates (Oxoid, Basingstoke, UK). Using both the genus- and species-specific PCR methods, 43 strains were identified as Lactobacillus spp., and 10 strains were confirmed as Lb. plantarum. First, the whole bacterial cultures of Lb. plantarum strains were tested by disc diffusion assay. All showed very good antibacterial activities against 6 selected foodborne pathogens, including Escherichia coli, Salmonella Enteritidis, Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus. Then, cell-free neutralized supernatants and partially purified bacteriocins were prepared from the 4 Lb. plantarum strains that exhibited the best antibacterial potential, and they were tested the same way as the whole bacterial cultures. Seven of the 10 Lb. plantarum strains harbored the plnEF gene, 3 strains harbored the plnD gene, and 2 strains possessed both the plnA and plnC genes that encode the production of the respective plantaricins. The presence of both plnR and plnL genes was only detected in a single Lb. plantarum isolate. Based on the results of this study, 4 strains of Lb. plantarum appeared to be suitable candidates for further testing in the dairy manufacturing sector, particularly in the production of raw sheep milk products.

这项研究的目的是筛选从传统的斯洛伐克生羊奶奶酪分离的植物乳杆菌菌株的抑制潜力。通过接种de Man，Rogosa和Sharpe琼脂板（Oxoid，Basingstoke，英国）。使用属和种特异性PCR方法，鉴定出43个菌株为乳酸杆菌属，确认10个菌株为Lb。车前草。首先是Lb的整个细菌培养。车前草通过盘扩散测定法测试菌株。所有这些都对6种选定的食源性病原体表现出非常好的抗菌活性，包括大肠杆菌，肠炎沙门氏菌，铜绿假单胞菌，单核细胞增生性李斯特菌，金黄色葡萄球菌和蜡样芽孢杆菌。然后，从4 Lb制备无细胞的中和上清液和部分纯化的细菌素。表现出最佳抗菌潜能的植物乳杆菌菌株，其测试方法与整个细菌培养物相同。10磅中的七个。杆菌菌株窝藏plnEF基因，3株窝藏plnD基因和2个菌株同时具有plnA和plnC基因，它们分别编码各自的植物霉素。仅在单个Lb中检测到了plnR和plnL基因。车前草分离物。根据这项研究的结果，有4株Lb。植物乳霜似乎是在乳制品制造业，特别是在生羊奶产品生产中进行进一步测试的合适候选者。