Betacyanins, natural plant pigments, from Iresine herbstii Hook. ex Lindl. leaf extract were separated for the first time by high-speed countercurrent chromatography (HSCCC) in a highly polar solvent system composed of PrOH–ACN–(NH₄)₂SO_4satd.soln–H₂O (1.0:0.5:1.2:1.0; v/v/v/v) in the tail-to-head mode. The flow rate of the mobile phase was 2.0 ml/min and the column rotation speed was 860 rpm. The retention of the stationary phase was 81.0%. For the identification of separated betacyanins in the crude extract and in the HSCCC fractions, as well as for the molecular formulas and multi-step fragmentation pattern elucidation, liquid chromatography with tandem mass spectrometry and high-resolution ion-trap time-of-flight mass spectrometry were performed.

The innovative application of the HSCCC system for fractionation of the betacyanins present in I. herbstii leaves enabled effective separation as well as preconcentration of the pigments for further low- and high-resolution LC-MS/MS analysis. HSCCC separation enabled identification of 22 betacyanins, of which 18 had not been detected previously in the leaves of I. herbstii, and four of these betacyanins (sinapoyl-gomphrenin and coumaroyl-gomphrenin as well as their epimers) were identified for the first time in the Iresine genus.

甜菜青素，天然植物色素，得自Iresine herbtii Hook。前林德尔。在由PrOH–ACN–（NH ₄）₂ SO _{4饱和–sol 2} –H ₂ O（1.0：0.5：1.2：1.0 ）组成的高极性溶剂体系中，通过高速逆流色谱（HSCCC）首次分离叶提取物; v / v / v / v）。流动相的流速为2.0 ml / min，色谱柱转速为860 rpm。固定相的保留率为81.0％。用于鉴定粗提物和HSCCC馏分中分离出的甜菜碱，以及分子式和多步裂解模式的阐明，液相色谱-串联质谱和高分辨离子阱飞行时间质谱进行光谱分析。

该系统HSCCC中存在的甜菜青素的分馏的创新应用I. herbstii叶启用进一步的低和高分辨率LC-MS / MS分析有效的分离，以及颜料的预浓缩。HSCCC分离启用的22个甜菜青素，其中18以前没有在叶检测的识别I. herbstii，并且这些甜菜青素的四（芥子酰-gomphrenin和香豆酰基gomphrenin以及它们的差向异构体）被确定为在第一时间在血苋属属。