Carbapenem-resistant Acinetobacter baumannii (CRAB) and Pseudomonas aeruginosa (CRPA), as well as polymyxin-resistant A. baumannii (PMR-AB) and P. aeruginosa (PMR-PA), were used to test different enrichment strategies from spiked stools. Three procedures were compared, namely, direct inoculation on selective plates and plating after a 24-h enrichment step in tryptic soy broth with or without antibiotics. Selective agar plates were used including CHROMagar-Pseudomonas supplemented with meropenem (2 mg/L), and CHROMagar-MDR-Acinetobacter agar and CHROMagar COL-APSE plates. Use of enrichment broths significantly enhanced the recovery of CRAB, CRPA, PMR-AB, and PMR-PA. However, supplementing or not the pre-enrichment broth with antibiotics had no impact. The proposed strategy for screening multidrug-resistant nonfermenters is of low cost, is easy to implement, and might be useful for outbreak containment.

耐碳青霉烯的鲍曼不动杆菌（CRAB）和铜绿假单胞菌（CRPA）以及耐多粘菌性的鲍曼不动杆菌（PMR-AB）和铜绿假单胞菌（PMR-PA）用于测试加标粪便的不同富集策略。比较了三种方法，即在选择性平板上直接接种和在有或没有抗生素的胰蛋白酶大豆肉汤中进行24小时富集步骤后进行平板接种。使用选择性琼脂平板，包括CHROMagar-假单胞菌补充美罗培南（2 mg / L），CHROMagar-MDR-不动杆菌琼脂和CHROMagar COL-APSE板。浓缩肉汤的使用显着增强了CRAB，CRPA，PMR-AB和PMR-PA的回收率。但是，在富集前肉汤中添加或不添加抗生素都没有影响。所提出的筛选耐多药非发酵剂的策略成本低廉，易于实施，并且可能有助于控制疫情。