Emerging nano-donor and nano-receptor FRET pairs are more advantageous to broad biosensing applications. However, those reported FRET system generally containing a single pair cannot afford multiplexed analysis. Herein, bi-color FRET from two nano-donors to a single nano-acceptor has been adopted to fabricate a simple-to-use aptasensor for simultaneous determination of dual targets in a single run. Aflatoxin B1 (AFB1) and ochratoxin A (OTA) were selected as the analytes because they have synergistic effect to induce enhanced toxicity. Specially, carbon dots (CDs) and CdZnTe quantum dots (QDs), with unambiguous separation of emission peaks, were used as nano-donors to label the aptamers specific for AFB1 and OTA, respectively. These single-stranded aptamers with labels could be stably adsorbed on a single nano-acceptor MoS₂ nanosheets surface due to van der Waals force, leading to the occurrence of FRET. When the aptasensor incubated with AFB1 and OTA, the specific binding between aptamers and targets resulted in partial release of the nano-donor labeled aptamers which disturb the FRET process and ultimately led to the fluorescence recovery to some extent. This aptasensor exhibited a wide detection range of 0.01–10 ng mL⁻¹ for AFB1 and 0.02–5 ng mL⁻¹ for OTA with low detection limits (3.3 pg mL⁻¹ for AFB1 and 7.1 pg mL⁻¹ for OTA). This aptasensor provides a new avenue for simultaneous determination of dual mycotoxins due to its advantages of simple operation, good selectivity and high sensitivity. By simply changing the specific aptamer, our strategy will certainly provide a universal aptasensing platform in advancing multiplexed analysis.

新兴的纳米供体和纳米受体FRET对更广泛的生物传感应用更为有利。但是，那些报道的FRET系统通常只包含一对，无法进行多重分析。在本文中，已采用从两个纳米供体到单个纳米受体的双色FRET来制造简单易用的适体传感器，以便在一次运行中同时确定双靶标。选择黄曲霉毒素B1（AFB1）和och曲霉毒素A（OTA）作为分析物，因为它们具有协同作用以诱导增强的毒性。特别地，具有明确分离发射峰的碳点（CD）和CdZnTe量子点（QD）被用作纳米供体，分别标记了AFB1和OTA特有的适体。这些带有标记的单链适体可以稳定地吸附在单个纳米受体MoS _2上范德华力导致纳米片表面变形，从而导致FRET的发生。当将适体传感器与AFB1和OTA孵育时，适体与靶标之间的特异性结合会导致纳米级供体标记的适体的部分释放，这会干扰FRET过程，并最终在某种程度上导致荧光恢复。这种适体传感器表现出0.01〜10纳克mL的检测范围宽^-1为AFB 1和0.02-5纳克毫升^-1为OTA具有低检测限（3.3皮克毫升^-1为AFB 1和7.1皮克毫升^-1OTA）。该适体传感器具有操作简单，选择性好和灵敏度高的优点，为同时测定双霉菌毒素提供了新的途径。通过简单地更改特定的适体，我们的策略一定会为推进多元分析提供一个通用的适体平台。