P. stutzeri strain XL-2 initially immobilized on polypropylene carriers accelerated the biofilm formation in start-up of sequencing batch biofilm reactor (SBBR) (denoted R1). The biofilm formation in R1 was approximately completed in 36 days, which was shorter than that of 48 days in an identical SBBR (denoted R2) without strain XL-2. Meanwhile, R1 presented a rapid stabilization of NH₄⁺-N and TN removal to 81.7% and 72.4% respectively. Surface plasmon resonance demonstrated that strain XL-2 enhanced the initial adhesion of carrier surface due to the production of extracellular polymeric substances (EPS), which made it easier for other EPS-producing strains, such as Thauera and Flavobacterium, to adhere to the carriers. PICRUSt revealed that biofilm in R1 presented relatively higher activity of EPS biosynthesis enzymes (glycosyltransferase and asparagine synthase). Thus, high EPS content was obtained due to the application of carriers immobilized with strain XL-2 and finally promoted the biofilm formation.

P。最初固定在聚丙烯载体上的stutzeri菌株XL-2在启动测序分批生物膜反应器（SBBR）（表示为R1）时加速了生物膜的形成。R1中的生物膜形成大约在36天内完成，这比没有菌株XL-2的相同SBBR（表示为R2）中的48天短。同时，R1使NH ₄ ⁺ -N和TN的去除快速稳定，分别达到81.7％和72.4％。表面等离子体共振表明，XL-2菌株由于产生细胞外聚合物（EPS）而增强了载体表面的初始粘附力，这使得其他产生EPS的菌株（如Thauera和Flavobacterium）更容易，坚持承运人。PICRUSt揭示，R1中的生物膜表现出相对较高的EPS生物合成酶（糖基转移酶和天冬酰胺合成酶）活性。因此，由于施加了用菌株XL-2固定的载体而获得了高EPS含量，并最终促进了生物膜的形成。