This study investigated synthetic wastewater treatment under low inflow C/N ratio and characterized NO₃⁻–N-transforming and electricity-producing bacteria in a multi-anode tidal constructed wetland–microbial fuel cell (TFCW–MFC). The optimal concurrent average removal rates of NH₄⁺–N and NO₃⁻–N were 73% and 78%, respectively, under a flood/rest/flood time of 4 h/2h/4h in “tide” mode accompanied by one recirculation. The lowest NO₃⁻–N concentration among all anodes was observed when the electrode gap was 45 cm. Similarly, the 45 cm anode exhibited selective enrichment of Variovorax and Azoarcus. Correction analysis showed that the high relative abundance of Azoarcus was crucial in enhancing NO₃⁻–N removal, and the internal resistance significantly decreased as the relative abundance of Acidovorax increased. These results suggest that NO₃⁻–N removal and bioelectricity generation can be promoted in a TFCW–MFC with limited carbon by improving the culture conditions for specific genera.

这项研究调查了低流入C / N比下的合成废水处理，并表征了多阳极潮汐人工湿地微生物燃料电池（TFCW-MFC）中的NO ₃ ^-- N转化和发电细菌。在“潮汐”模式下，在4 h / 2h / 4h的淹水/休息/浸水时间下，NH ₄ ⁺ -N和NO ₃ ^-- N的最佳同时平均去除率分别为73％和78％。再循环。当电极间隙为45 cm时，观察到所有阳极中最低的NO ₃ ^-- N浓度。同样，在45厘米长的阳极上，选择性地富集了Variovorax和Azoarcus。校正分析表明，较高的固氮菌相对丰度对增强NO ₃ ^-- N的去除至关重要，并且随着抗酸菌相对丰度的增加，内阻显着降低。这些结果表明，通过改善特定属的培养条件，可以在碳含量有限的TFCW-MFC中促进NO ₃ ^-- N的去除和生物电的产生。