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Genetic polymorphism in IL17RA induces susceptibility to Toxoplasma gondii infection in Brazilian pregnant women.
Acta Tropica ( IF 2.1 ) Pub Date : 2020-06-26 , DOI: 10.1016/j.actatropica.2020.105594
Joelma Maria de Araujo Andrade 1 , Claudio Bruno Silva de Oliveira 2 , Ywlliane da Silva Rodrigues Meurer 3 , Jéssica Emanuella Santana 4 , Yngrid Gleyter Barbosa de Almeida 4 , Priscilla Vilela Dos Santos 5 , Débora Maria Soares de Souza 5 , Guilherme de Paula Costa 5 , André Talvani 5 , Gustavo Martelli Palomino 6 , Janaina Cristiana de Oliveira Crispim Freitas 6 , Valter Ferreira de Andrade-Neto 2
Affiliation  

Congenital toxoplasmosis is a parasitic disease caused by Toxoplasma gondii, an obligate intracellular parasite which can cause fetal death/abortion and can induce damage in the brain and eyes of the infected babies. The environmental and genetic factors associated with T. gondii and the maternal immune response, drive part of the pathogenesis of congenital toxoplasmosis. Thus, in this study, we aimed to investigate the allelic and genotypic frequencies of specific single nucleotide polymorphisms (SNPs) in the IL17A and IL17RA genes, as well as the production of IL-17A, IL-33, and CCL2 in pregnant women, from the State of Rio Grande do Norte, Brazil, further relating these along with the clinical parameters, to the toxoplasmosis infection. Through PCR-RFLP techniques, two SNPs implicated in Th17 immune response, IL17A rs2275913 (G> A) and IL17RA rs4819554 (A> G) modulation were evaluated in pregnant women, either infected or not infected by T. gondii. These women were also evaluated in terms of plasma release of CCL2, IL-33, and IL-17A which relate to hypertension, number of abortions, and ethnic pattern. The results showed that the G-allele of the SNP rs2275913 (IL17A) appeared to be protective in this population, while the rs4819554 (IL17RA) SNP G allele was associated with greater susceptibility to T. gondii infection [ρ value = 0.025; OR = 2.815 (1.118–7.089); CI = 95%]. None of the cytokines had any influence on the analyzed parameters (abortion and hypertension). In conclusion, our data suggest an immunogenic evidence of susceptibility to T. gondii infection driven by the rs4819554 (IL17RA) SNP G allele in Brazilian pregnant women. Further studies are needed to reinforce this trial marker in populations from distinct geographical areas as well as to confirm the protective pattern related to the G-allele of the SNP rs2275913 (IL17A) in pregnant women.



中文翻译:

IL17RA中的遗传多态性诱导巴西孕妇弓形虫感染的易感性。

先天性弓形虫病是一种由弓形虫(弓形虫)引起的寄生虫病,弓形虫是一种专性的细胞内寄生虫,可引起胎儿死亡/流产,并可能对受感染婴儿的大脑和眼睛造成伤害。与弓形虫和孕妇免疫反应有关的环境和遗传因素,驱动了先天性弓形虫病的发病机理。因此,在这项研究中,我们旨在研究IL17AIL17RA中特定单核苷酸多态性(SNP)的等位基因和基因型频率来自巴西北里奥格兰德州的孕妇基因,以及IL-17A,IL-33和CCL2的产生,将这些基因以及临床参数与弓形体感染进一步联系起来。通过PCR-RFLP技术,在感染或未感染弓形虫的孕妇中评估了两个与Th17免疫应答有关的SNP,即IL17A rs2275913(G > A)和IL17RA rs4819554(A > G)调节。还对这些妇女进行了血浆CCL2,IL-33和IL-17A释放的评估,这些血浆释放与高血压,流产次数和种族模式有关。结果显示SNP rs2275913(IL17A)在该人群中似乎是保护性的,而rs4819554(IL17RA)SNP G等位基因与刚地弓形虫感染的敏感性更高[ρ值= 0.025;或= 2.815(1.118–7.089);CI = 95%]。没有一种细胞因子对所分析的参数(流产和高血压)有任何影响。总而言之,我们的数据表明在巴西孕妇中,rs4819554(IL17RA)SNP G等位基因驱动的弓形虫感染易感性的免疫原性证据。需要进一步的研究以加强来自不同地理区域人群的这一试验标记,并确认与SNP rs2275913(IL17A) G等位基因相关的保护模式)在孕妇中。

更新日期:2020-07-06
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