The aims of this study were to investigate the chemical composition, in-vitro antioxidant and antibacterial activities of Syzygium aromaticum essential oil. The essential oil was obtained by hydrodistillation. The GC–MS/GC–FID was used for determination of essential oil composition. The antioxidant activity was determined by DPPH radical scavenging assay and reducing power. The antimicrobial activity was evaluated by the broth microdilution method against six enteropathogens bacteria isolated from diarrhea of young calves. The results of the study revealed an average yield of the essential oil of 11.6% ± 0.97 (w/w). About 65 components were identified and quantified. Eugenol was the major compound of the studied essential oil (78.72%) followed by β-caryophyllene (8.82%) and eugenyl acetate (8.74%). The antioxidant activity by DPPH assay showed IC50 value of 4.82 ± 0.06 × 10⁻² µg/mL, while the reducing power assay have resulted an EC50 value of 3.47 ± 0.2 × 10⁻² µg/mL. The results showed that the essential oil exhibited an MIC at 1.36 mg/mL to 2.72 mg/ml and MBC at 5.45 mg/mL to 10.9 mg/mL against all tested bacteria. The present investigation revealed that Syzygium aromaticum essential oil exhibited a good in-vitro antioxidant and moderate in-vitro antibacterial activities.

这项研究的目的是研究化学成分，体外抗氧化和抗菌活性的丁香香精油。通过加氢蒸馏获得精油。GC-MS / GC-FID用于测定精油成分。抗氧化剂活性通过DPPH自由基清除试验和还原能力确定。通过肉汤微量稀释法评价了对从幼小牛腹泻中分离出的六种肠病原菌的抗菌活性。研究结果显示，精油的平均收率为11.6％±0.97（w / w）。鉴定并定量了约65种成分。丁香酚是所研究精油的主要化合物（78.72％），其次是β-石竹烯（8.82％）和乙酸丁二烯酯（8.74％）。DPPH测定的抗氧化活性显示IC50值为4.82±0.06×10 ^-2µg / mL，而还原力测定得出的EC50值为3.47±0.2×10 ^-2 µg / mL。结果表明，精油对所有测试细菌的MIC为1.36 mg / mL至2.72 mg / ml，MBC为5.45 mg / mL至10.9 mg / mL。本研究表明，蒲桃精油具有良好的体外抗氧化剂和适度的体外抗菌活性。