Seaweed-derived compounds are known to possess various biological activities. The present study evaluated the antioxidant potential of (−)-loliolide, a monoterpenoid isolated from Sargassum horneri in Vero cells and zebrafish models. (−)-Loliolide was purified from the chloroform fraction of an S. horneri methanol extract using high-performance centrifugal partition chromatography (HPCPC). HPCPC was found to be an effective method to isolate (−)-loliolide, yielding a high purity product. Electron spin resonance evaluation demonstrated that (−)-loliolide had significant alkyl radical scavenging activity (IC₅₀: 0.043 ± 0.005 mg mL⁻¹). In addition, our study showed that (−)-loliolide demonstrated dose-dependent protective effects for Vero cell viability against 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced intracellular reactive oxygen species (ROS). Moreover, (−)-loliolide reduced lipid peroxidation in AAPH-induced zebrafish embryos, while ameliorating the survival rate and cell death in a dose-dependent manner. These results reveal the antioxidant potential of (−)-loliolide. Future studies highlighting the potential applications of S. horneri in manufacturing functional food ingredients are required.

已知海藻衍生的化合物具有多种生物活性。本研究评估了（-）-loliolide的抗氧化潜力，这是一种在Vero细胞和斑马鱼模型中从Sargassum horneri分离出的单萜类化合物。（ - ） - Loliolide是从一个的氯仿级分纯化S. horneri甲醇提取物采用高性能离心分配色谱法（HPCPC）。已发现HPCPC是分离（-）-卤代内酯的有效方法，可产生高纯度的产物。电子自旋共振评估表明（-）-氟碘化物具有明显的烷基清除活性（IC ₅₀：0.043±0.005 mg mL ^-1）。此外，我们的研究表明，（-）-loliolide具有针对2,2'-偶氮双（2-ami基丙烷）二盐酸盐（AAPH）诱导的细胞内活性氧（ROS）的Vero细胞活力剂量依赖性保护作用。此外，（-）-loliolide可减少AAPH诱导的斑马鱼胚胎中的脂质过氧化，同时以剂量依赖的方式改善存活率和细胞死亡。这些结果表明（-）-氟碘化物的抗氧化潜力。需要进行进一步的研究，以突出霍恩沙棘在生产功能性食品成分中的潜在应用。