Alkaline hydrogen peroxide (AHP) pretreatment is a promising process for enhancing enzymatic digestibility of lignocellulosic biomass in biorefineries. In the present work, the effects of organic bases (NH₄OH and tri-ethylamine) and co-solvents (ethanol, isopropanol, tert-butyl alcohol, water) on AHP pretreatment efficiency of rice straw were studied and compared to the typical aqueous reaction with NaOH. It was found that the glucose recovery from enzymatic hydrolysis of the biomass pretreated by AHP at 35 °C for 24 h using NH₄OH in aqueous/tert-butyl alcohol (73.6%) was higher than that achieved using ethanol and isopropanol (31.6–48.6%) and water (71.2%) under the same experimental conditions. Increasing H₂O₂ concentration from 1 to 10% v/v in the aqueous/tert-butyl alcohol with NH₄OH led to enhancing sugar yield to from 349 to 623 mg/g pretreated rice straw, equivalent to the highest glucose recovery of 83.0%. Formation of highly porous structures in pretreated rice straw by removals of hemicelluloses and lignin was revealed by Fourier transform infrared spectroscopy and scanning electron microscopy while the increased crystallinity index was shown by X-ray diffraction. This modified low-temperature AHP pretreatment using organic solvent system is advantageous on recyclability potential of the reagents and potent for further implementation in lignocellulosic biorefineries.

碱性过氧化氢（AHP）预处理是在生物精炼厂中增强木质纤维素生物质的酶消化率的有前途的方法。在本工作中，研究了有机碱（NH ₄ OH和三乙胺）和助溶剂（乙醇，异丙醇，叔丁醇，水）对稻草AHP预处理效率的影响，并将其与典型的水与NaOH反应。发现在水/叔丁醇中使用NH ₄ OH （35.6％）在NH ₄ OH的条件下于35°C进行AHP预处理24 h的酶水解产生的葡萄糖回收率高于使用乙醇和异丙醇（31.6–在相同的实验条件下为48.6％）和水（71.2％）。H ₂增加在含NH ₄ OH的叔丁醇水溶液中，O _2的浓度从1％到10％v / v，可将糖的产量提高到349至623 mg / g预处理的稻草，相当于最高的葡萄糖回收率为83.0％。傅里叶变换红外光谱和扫描电镜观察表明，通过半纤维素和木质素的去除在预处理的稻草中形成了高度多孔的结构，而通过X射线衍射显示出结晶度指数的增加。使用有机溶剂系统进行的这种改进的低温AHP预处理有利于试剂的可回收利用潜力，并且对于进一步在木质纤维素生物精炼厂中实施具有潜力。