Background

Dirofilaria immitis and Dirofilaria repens are the causative agents of cardiopulmonary and subcutaneous dirofilariosis, respectively. This neglected disease mainly seen in dogs, cats and wild carnivores is re-emerging recent years. No study was conducted on dirofilariosis in dogs in Kyrgyzstan.

Purpose

The goal of this study was to investigate Dirofilaria species using PCR and sequencing in dogs from Kyrgyzstan.

Method

Dirofilaria spp. infection in dogs was screened via convential PCR and sequencing in 337 dogs from Kyrgyzstan.

Result

The overall prevalence of Dirofilaria spp. was 0.59% (2/337): DNA of D. immitis was detected in one sample and DNA of D. repens in second positive sample. In second sample, parallel co-infection of D. repens with Wolbachia was also found. While D. immitis sequence showed 98.70–100% similarity with previously reported sequences of D. immitis from dog blood, D. repens shared 100% identity with other sequences of D. repens.

Conclusion

These results provided first evidence for Dirofilaria spp. in Kyrgyzstan and emphasized the veterinary and medical importance.

中文翻译：

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吉尔吉斯斯坦犬犬恶丝虫和白线虫的首次分子检测。

背景

Dirofilaria immitis和Dirofilaria repens分别是心肺和皮下恶丝虫病的病原体。这种被忽视的疾病主要见于狗、猫和野生食肉动物，近年来又重新出现。吉尔吉斯斯坦没有对狗的恶丝虫病进行研究。

目的

本研究的目的是在吉尔吉斯斯坦的狗中使用 PCR 和测序来调查恶丝虫种类。

方法

恶丝虫属 通过常规 PCR 和测序对来自吉尔吉斯斯坦的 337 只狗进行了筛选。

结果

Dirofilaria spp的总体流行率。为 0.59% (2/337)：在 1 个样本中检测到D. immitis 的DNA，在第二个阳性样本中检测到D. repens 的DNA 。在第二个样本中，还发现了白蜡木与Wolbachia 的平行共感染。虽然D.丝虫序列显示出与先前报道的序列98.70-100％的相似D.丝虫从犬血液，马蹄金共享100％的同一性与其他序列马蹄金。

结论

这些结果为Dirofilaria spp提供了第一个证据。在吉尔吉斯斯坦，并强调了兽医和医学的重要性。