Efficient biotransformation of 5-hydroxymethylfurfural to 5-hydroxymethyl-2-furancarboxylic acid by a new whole-cell biocatalyst Pseudomonas aeruginosa PC-1,Reaction Chemistry & Engineering

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Efficient biotransformation of 5-hydroxymethylfurfural to 5-hydroxymethyl-2-furancarboxylic acid by a new whole-cell biocatalyst Pseudomonas aeruginosa PC-1
Reaction Chemistry & Engineering ( IF 3.4 ) Pub Date : 2020-06-24 , DOI: 10.1039/d0re00018c
Xin Pan _{1,

2,

3,

4,

5} , Sihua Wu _{1,

2,

3,

4,

5} , Deshan Yao _{1,

2,

3,

4,

5} , Lian Liu _{1,

2,

3,

4,

5} , Lina Zhang _{1,

2,

3,

4,

5} , Zixuan Yao _{5,

6,

7,

8} , Yan Pan _{5,

6,

7,

8} , Siyuan Chang _{5,

6,

7,

8} , Bingfeng Li _{5,

6,

7,

8}

Affiliation

5-Hydroxymethyl-2-furancarboxylic acid (HMFCA) is a significant added-value intermediate derivatized from the bio-based platform chemical 5-hydroxymethylfurfuraldehyde (HMF). In the HMFCA biosynthesis process, the catalytic performance of the biocatalyst always decreases due to the presence of HMF toxicity, which is not conducive to achieving high product accumulation. Herein, we successfully obtained a new whole-cell biocatalyst from Pseudomonas aeruginosa PC-1, which was first reported to produce HMFCA from HMF. After optimization, 90.1% yield of HMFCA was attained within 6 h using a 100 mM substrate. Importantly, a much lower cell dosage was needed in the process. In order to achieve higher HMFCA accumulation, a fed-batch strategy was developed by using the growing cells from P. aeruginosa PC-1. Consequently, a total concentration of 721 mM HMFCA was obtained from 800 mM HMF within 58 h after 8 cycles of fed-batch, which is the highest concentration to date. The efficient HMFCA production made P. aeruginosa PC-1 a promising whole-cell biocatalyst for selective, large scale biotransformation of HMF.

中文翻译：

新型全细胞生物催化剂铜绿假单胞菌PC-1将5-羟甲基糠醛高效生物转化为5-羟甲基-2-呋喃甲酸

5-羟甲基-2-呋喃甲酸（HMFCA）是衍生自生物基平台化学5-羟甲基糠醛（HMF）的重要增值中间体。在HMFCA生物合成过程中，由于HMF毒性的存在，生物催化剂的催化性能总是下降，这不利于实现高产物积聚。在这里，我们成功地从铜绿假单胞菌PC-1成功获得了一种新的全细胞生物催化剂，据报道，它最初是由HMF生产HMFCA。优化后，使用100 mM底物，在6小时内HMFCA的收率为90.1％。重要的是，在该过程中需要低得多的细胞剂量。为了实现更高的HMFCA积累，通过使用来自铜绿假单胞菌的生长细胞开发了补料分批策略PC-1。因此，在8批分批补料后58小时内，从800 mM HMF中获得了721 mM HMFCA的总浓度，这是迄今为止最高的浓度。高效的HMFCA生产使铜绿假单胞菌PC-1成为用于HMF选择性大规模生物转化的有前途的全细胞生物催化剂。

更新日期：2020-07-28

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