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Why synthetic virus-like nanoparticles can achieve higher cellular uptake efficiency?
Nanoscale ( IF 5.8 ) Pub Date : 2020-06-25 , DOI: 10.1039/d0nr03234d Jiawei Li 1 , Junfeng Wang 2 , Qiang Yao 2 , Tao Li 3 , Youguo Yan 4 , Zhen Li 2 , Jun Zhang 4
Nanoscale ( IF 5.8 ) Pub Date : 2020-06-25 , DOI: 10.1039/d0nr03234d Jiawei Li 1 , Junfeng Wang 2 , Qiang Yao 2 , Tao Li 3 , Youguo Yan 4 , Zhen Li 2 , Jun Zhang 4
Affiliation
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Experimental studies in recent years have demonstrated that the cellular uptake properties of nanoparticles can be improved by mimicking the spiky surfaces of viruses; however, little is known on how the surface topological structure of nanoparticles affects their translocation across the cell membrane. Here, by employing dissipative particle dynamics simulations, the interactions between virus-like nanoparticles (VLPs) and the lipid bilayer are investigated. The analysis of critical force for penetration demonstrates that VLPs with relatively longer and sparser spikes have better penetrability. The internalization pathway of VLPs illustrates that the spikes of VLPs can perturb the bilayer structure after VLPs adhere onto the bilayer. Furthermore, by comparing the translocation process of VLPs and spherical nanoparticles, it is found that the presence of spikes can help to increase the lateral defects in the bilayer, decrease the vertical deformation of the bilayer, and lower the density of nearby lipids during the translocation process. These effects of spikes jointly contribute to the superior penetrability of VLPs. It is expected that these findings not only enrich our understanding of how the surface topological structure affects the cellular uptake, but also pave the way for further development of VLPs for versatile biomedical applications.
中文翻译：
为什么合成病毒样纳米颗粒可以实现更高的细胞吸收效率？
近年来的实验研究表明，通过模仿病毒的尖刺表面可以改善纳米颗粒的细胞摄取特性。然而，关于纳米颗粒的表面拓扑结构如何影响其跨细胞膜转运的了解甚少。在这里，通过采用耗散粒子动力学模拟，研究了病毒样纳米颗粒（VLP）与脂质双层之间的相互作用。对穿透的临界力的分析表明，具有相对较长且较稀疏的尖峰的VLP具有更好的渗透性。VLP的内部化途径说明，在VLP粘附到双层之后，VLP的尖峰会干扰双层结构。此外，通过比较VLP和球形纳米颗粒的转运过程，已发现尖峰的存在可以帮助增加双层中的侧向缺陷，减少双层的垂直变形并降低易位过程中附近脂质的密度。尖峰的这些影响共同有助于VLP的出色渗透性。预期这些发现不仅可以丰富我们对表面拓扑结构如何影响细胞摄取的理解，而且可以为进一步开发多功能生物医学应用的VLPs铺平道路。
更新日期：2020-07-16
中文翻译：
为什么合成病毒样纳米颗粒可以实现更高的细胞吸收效率？
近年来的实验研究表明，通过模仿病毒的尖刺表面可以改善纳米颗粒的细胞摄取特性。然而，关于纳米颗粒的表面拓扑结构如何影响其跨细胞膜转运的了解甚少。在这里，通过采用耗散粒子动力学模拟，研究了病毒样纳米颗粒（VLP）与脂质双层之间的相互作用。对穿透的临界力的分析表明，具有相对较长且较稀疏的尖峰的VLP具有更好的渗透性。VLP的内部化途径说明，在VLP粘附到双层之后，VLP的尖峰会干扰双层结构。此外，通过比较VLP和球形纳米颗粒的转运过程，已发现尖峰的存在可以帮助增加双层中的侧向缺陷，减少双层的垂直变形并降低易位过程中附近脂质的密度。尖峰的这些影响共同有助于VLP的出色渗透性。预期这些发现不仅可以丰富我们对表面拓扑结构如何影响细胞摄取的理解，而且可以为进一步开发多功能生物医学应用的VLPs铺平道路。
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