The bacterial community present in the abdomen in Anophelinae mosquitoes can influence mosquito susceptibility to Plasmodium infection. Little is known about the bacteria associated with Nyssorhynchus darlingi, a primary malaria vector in the Amazon basin. We investigated the abdominal bacterial community compositions of naturally Plasmodium-infected (P-positive, n = 9) and non-infected (P-negative, n = 7) Ny. darlingi from the Brazilian Amazon region through massive parallel sequencing of the bacterial V4 variable region of the 16S rRNA gene. Bacterial richness of Ny. darlingi encompassed 379 operational taxonomic units (OTUs), the majority of them belonging to the Proteobacteria, Firmicutes and Bacteroides phyla. Escherichia/Shigella and Pseudomonas were more abundant in the P-positive and P-negative groups, respectively, than in the opposite groups. Enterobacter was found only in the P-negative group. The results of statistical analyses conducted to compare bacterial abundance and diversity between Plasmodium-infected and Plasmodium-non-infected mosquitoes were not significant. This study increased knowledge about bacterial composition in Ny. darlingi and revealed that Plasmodium-positive and Plasmodium-negative groups share a common core of bacteria. The genera Prevotella 9, Sphingomonas, Bacteroides, and Bacillus were reported for the first time in Ny. darlingi.

中文翻译：

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与天然疟原虫感染和未感染的Nyssorhynchus darlingi腹部相关的细菌多样性。

蚊虫腹部存在的细菌群落可影响蚊虫对疟原虫感染的易感性。关于与Nyssorhynchus darlingi（亚马逊河流域的主要疟疾媒介）有关的细菌知之甚少。我们调查了天然疟原虫感染（P阳性，n = 9）和未感染（P阴性，n = 7）Ny的腹部细菌群落组成。通过对16S rRNA基因的细菌V4可变区进行大规模平行测序，从巴西亚马逊地区获得darlingi。Ny的细菌丰富性。darlingi包含379个操作生物分类单位（OTU），其中大多数属于变形杆菌，硬毛菌和门生细菌门。大肠埃希菌/志贺氏菌和假单胞菌分别在P阳性和P阴性组中更为丰富，比在相反的群体。仅在P阴性组中发现了肠杆菌。进行统计学分析以比较感染疟原虫和未感染疟原虫的细菌丰度和多样性的结果并不显着。这项研究增加了有关Ny中细菌成分的知识。darlingi并揭示，疟原虫阳性和疟原虫阴性基团具有共同的细菌核心。Ny首次报道了Prevotella 9，鞘氨醇单胞菌，拟杆菌和芽孢杆菌属。达令吉。darlingi并揭示，疟原虫阳性和疟原虫阴性基团具有共同的细菌核心。Ny首次报道了Prevotella 9，鞘氨醇单胞菌，拟杆菌和芽孢杆菌属。达令吉。darlingi并揭示，疟原虫阳性和疟原虫阴性基团具有共同的细菌核心。Ny首次报道了Prevotella 9，鞘氨醇单胞菌，拟杆菌和芽孢杆菌属。达令吉。