Vibrio parahaemolyticus, an easy-ignored food-borne pathogen, can cause bacterial outbreaks and human disease during early-stage infection. In this study, we aimed to evaluate the detection efficiency of loop-mediated isothermal amplification (LAMP) as an emerging technique to directly detect V. parahaemolyticus infection in mammalian hosts and assess its potential in clinical applications. A LAMP assay was used for rapid identification of V. parahaemolyticus in a variety of mouse models in which animals were infected via the digestive tract, wounds, or through general infection, and the results were compared with routine analytical methods. Our results confirmed that the LAMP assay was capable of detecting V. parahaemolyticus in different mouse organs independent of the source of bacteria, although its sensitivity depended on the route of infection and the organ affected. Foodborne-derived V. parahaemolyticus was the most sensitive route, with the small intestine being the most sensitive organ. The LAMP assay indicated that V. parahaemolyticus that spread through the blood stream had the most serious consequences during early-stage infection. Positive LAMP results were identified in all blood samples from i.v. injected mice. Furthermore, the LAMP method could directly detect trace quantities of V. parahaemolyticus in fresh peripheral blood while conventional methods failed to do so, thereby shortening the time-to-result from days to minutes. In this study, we demonstrated that the LAMP assay was effective in speeding up the detection of V. parahaemolyticus. Instead of being a secondary method to assist in the clinic, the LAMP assay has potential for use as the primary technique for rapid detection of V. parahaemolyticus in the future.

中文翻译：

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使用LAMP方法在体内通过不同类型的手法快速鉴定和检测副溶血性弧菌

副溶血性弧菌是一种易于忽略的食源性病原体，可在早期感染期间引起细菌爆发和人类疾病。在这项研究中，我们旨在评估环介导的等温扩增（LAMP）的检测效率，这是一种直接检测哺乳动物宿主中副溶血性弧菌感染并评估其在临床应用中的潜力的新兴技术。在多种小鼠模型中，通过消化道，伤口或普通感染对动物进行了感染，LAMP测定法可用于快速鉴定副溶血性弧菌，并将其与常规分析方法进行比较。我们的结果证实，LAMP测定法能够检测不同小鼠器官中的副溶血性弧菌，而与细菌来源无关，尽管其敏感性取决于感染途径和受影响的器官。食源性副溶血性弧菌是最敏感的途径，小肠是最敏感的器官。LAMP分析表明，在早期感染期间，传播通过血流的副溶血性弧菌具有最严重的后果。在静脉注射的小鼠的所有血液样品中均鉴定出阳性LAMP结果。此外，LAMP方法可以直接检测新鲜外周血中的痕量溶血性弧菌，而常规方法则无法做到，从而将结果的时间从几天缩短至几分钟。在这项研究中，我们证明了LAMP分析可有效加快副溶血性弧菌的检测。而不是辅助临床的辅助方法，