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Simple and label-free strategy for terminal transferase assay using a personal glucose meter.
Chemical Communications ( IF 4.3 ) Pub Date : 2020-06-25 , DOI: 10.1039/d0cc02869j
Hyo Yong Kim 1 , Jayeon Song , Ki Soo Park , Hyun Gyu Park
Affiliation  

We herein developed a simple personal glucose meter (PGM)-based method for terminal transferase (TdT) activity assay by utilizing the glucose oxidase (GOx)-mimicking activity of cerium oxide nanoparticles (CeO2 NPs). Using this strategy, the TdT activity was reliably determined down to 0.7 U mL−1 with high selectivity against other non-specific enzymes.

中文翻译:

使用个人血糖仪进行末端转移酶测定的简单且无标签的策略。

我们在这里开发了一种简单的基于个人血糖仪(PGM)的方法,通过利用氧化铈纳米颗粒(CeO 2 NPs)的模仿葡萄糖氧化酶(GOx)的活性来进行末端转移酶(TdT)活性的测定。使用该策略,可以可靠地确定低至0.7 U mL -1的TdT活性,并且具有针对其他非特异性酶的高选择性。
更新日期:2020-08-06
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