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Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging analysis of endogenous metabolites in cherry tomatoes.
Analyst ( IF 3.6 ) Pub Date : 2020-06-25 , DOI: 10.1039/d0an00818d M Caleb Bagley 1 , Crystal L Pace 1 , Måns Ekelöf 1 , David C Muddiman 2
Analyst ( IF 3.6 ) Pub Date : 2020-06-25 , DOI: 10.1039/d0an00818d M Caleb Bagley 1 , Crystal L Pace 1 , Måns Ekelöf 1 , David C Muddiman 2
Affiliation
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We report the spatially resolved metabolic profiling of cherry tomatoes using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI), a mass spectrometry imaging (MSI) technique that operates at ambient conditions and requires no sample derivatization. Tomatoes were flash frozen, cryosectioned and imaged with adequate spatial resolution to distinguish between the major tissue structures of a tomato including the skin, mesocarp, endocarp, locular tissue, septum, placenta, seed and seed coating. Metabolites were imaged from 100–1200 m/z, enabling significant coverage of a diverse array of metabolites including amino acids and lipids along with the major secondary metabolite classes: terpenes, phenolics, glycosides, and alkaloids. During the metabolic profiling, we found endogenous carotenoid hydrocarbons, namely lycopene or its structural isomer β-carotene, ionized as radical cations. To our knowledge, this is the first demonstration of ionizing hydrocarbons in the MSI field.
中文翻译：
红外基质辅助激光解吸电喷雾电离 (IR-MALDESI) 质谱成像分析樱桃番茄的内源代谢物。
我们报告了使用红外基质辅助激光解吸电喷雾电离（IR-MALDESI）对樱桃番茄进行空间分辨代谢分析，这是一种在环境条件下运行且无需样品衍生化的质谱成像（MSI）技术。将番茄快速冷冻、冷冻切片并以足够的空间分辨率成像，以区分番茄的主要组织结构，包括皮肤、中果皮、内果皮、眼组织、隔膜、胎盘、种子和种皮。代谢物在 100–1200 m / z范围内成像，能够显着覆盖多种代谢物，包括氨基酸和脂质以及主要的次级代谢物类别：萜烯、酚类、糖苷和生物碱。在代谢分析过程中，我们发现内源性类胡萝卜素碳氢化合物，即番茄红素或其结构异构体β-胡萝卜素，以自由基阳离子形式电离。据我们所知，这是在 MSI 领域首次演示电离碳氢化合物。
更新日期：2020-08-10
中文翻译：
红外基质辅助激光解吸电喷雾电离 (IR-MALDESI) 质谱成像分析樱桃番茄的内源代谢物。
我们报告了使用红外基质辅助激光解吸电喷雾电离（IR-MALDESI）对樱桃番茄进行空间分辨代谢分析，这是一种在环境条件下运行且无需样品衍生化的质谱成像（MSI）技术。将番茄快速冷冻、冷冻切片并以足够的空间分辨率成像，以区分番茄的主要组织结构，包括皮肤、中果皮、内果皮、眼组织、隔膜、胎盘、种子和种皮。代谢物在 100–1200 m / z范围内成像，能够显着覆盖多种代谢物，包括氨基酸和脂质以及主要的次级代谢物类别：萜烯、酚类、糖苷和生物碱。在代谢分析过程中，我们发现内源性类胡萝卜素碳氢化合物，即番茄红素或其结构异构体β-胡萝卜素，以自由基阳离子形式电离。据我们所知，这是在 MSI 领域首次演示电离碳氢化合物。
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