pH-Controlled Detachable DNA Circuitry and Its Application in Resettable Self-Assembly of Spherical Nucleic Acids.,ACS Nano

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pH-Controlled Detachable DNA Circuitry and Its Application in Resettable Self-Assembly of Spherical Nucleic Acids.
ACS Nano ( IF 15.8 ) Pub Date : 2020-06-24 , DOI: 10.1021/acsnano.0c02329
Yijun Guo ₁ , Dongbao Yao ₁ , Bin Zheng ₂ , Xianbao Sun ₁ , Xiang Zhou ₁ , Bing Wei ₁ , Shiyan Xiao ₁ , Miao He ₁ , Chengxu Li ₁ , Haojun Liang ₁

Affiliation

Toehold-mediated strand displacement reaction, the fundamental basis in dynamic DNA nanotechnology, has proven its extraordinary power in programming dynamic molecular systems. Programmed activation of the toehold in a DNA substrate is crucial for building sophisticated DNA devices with digital and dynamic behaviors. Here we developed a detachable DNA circuit by embedding a pH-controlled intermolecular triplex between the toehold and branch migration domain of the traditional “linear substrate”. The reaction rate and the “on/off” state of the detachable circuit can be regulated by varying the pHs. Similarly, a two-input circuit composed of three pH-responsive DNA modules was then constructed. Most importantly, a resettable self-assembly system of spherical nucleic acids was built by utilizing the high detachability of the intermolecular triplex structure-based DNA circuit. This work demonstrated a dynamic DNA device that can be repeatedly operated at constant temperature without generating additional waste DNA products. Moreover, this strategy showed an example of recycling waste spherical nucleic acids from a self-assembly system of spherical nucleic acids. Our strategy will provide a facile approach for dynamic regulation of complex molecular systems and reprogrammable nanoparticle assembly structures.

中文翻译：

pH控制的可分离DNA电路及其在球形核酸可自组装中的应用。

脚趾介导的链置换反应是动态DNA纳米技术的基础，已证明其在编程动态分子系统中具有非凡的功能。DNA底物中的脚趾的程序激活对于构建具有数字和动态行为的复杂DNA设备至关重要。在这里，我们通过在传统“线性底物”的脚趾和分支迁移域之间嵌入pH控制的分子间三链体，从而开发了可分离的DNA电路。可以通过改变pH值来调节反应速率和可拆卸回路的“开/关”状态。同样，然后构造了由三个pH响应DNA模块组成的双输入电路。最重要的是，利用基于分子间三链体结构的DNA电路的高度可分离性，构建了球形核酸的可重置自组装系统。这项工作展示了一种动态DNA装置，该装置可以在恒定温度下重复操作，而不会产生其他废DNA产物。此外，该策略显示了从球形核酸自组装系统回收废弃球形核酸的一个例子。我们的策略将为复杂分子系统和可重编程纳米粒子组装结构的动态调节提供一种简便的方法。该策略显示了从球形核酸自组装系统回收废弃球形核酸的一个例子。我们的策略将为复杂分子系统和可重编程纳米粒子组装结构的动态调节提供一种简便的方法。该策略显示了从球形核酸自组装系统回收废弃球形核酸的一个例子。我们的策略将为复杂分子系统和可重编程纳米粒子组装结构的动态调节提供一种简便的方法。

更新日期：2020-07-28

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