Silicon (Si) supplementation has been shown to improve plant tolerance to different stresses, and its accumulation in the aerial organs is mediated by NIP2;1 aquaporins (Lsi channels) and Lsi2-type exporters in roots. In the present study, we tested the hypothesis that grapevine expresses a functional NIP2;1 that accounts for root Si uptake and, eventually, Si accumulation in leaves. Own-rooted grapevine cuttings of the cultivar Vinhão accumulated >0.2% Si (DW) in leaves when irrigated with 1.5 mM Si for 1 month, while Si was undetected in control leaves. Real-time PCR showed that VvNIP2;1 was highly expressed in roots and in green berries. The transient transformation of tobacco leaf epidermal cells mediated by Agrobacterium tumefaciens confirmed VvNIP2;1 localization at the plasma membrane. Transport experiments in oocytes showed that VvNIP2;1 mediates Si and arsenite uptake, whereas permeability studies revealed that VvNIP2;1 expressed in yeast is unable to transport water and glycerol. Si supplementation to pigmented grape cultured cells (cv. Gamay Freáux) had no impact on the total phenolic and anthocyanin content, or on the growth rate and VvNIP2;1 expression. Long-term experiments should help determine the extent of Si uptake over time and whether grapevine can benefit from Si fertilization.

中文翻译：

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VvNIP2; 1水通道蛋白是葡萄硅通道。

硅（Si）补充剂已显示可提高植物对不同胁迫的耐受性，其在气生器官中的积累是由NIP2; 1水通道蛋白（Lsi通道）和根部的Lsi2型输出介导的。在本研究中，我们测试了葡萄藤表达功能性NIP2; 1的假说，该功能解释了根部Si的吸收以及最终Si在叶片中的积累。用1.5 mM Si灌溉1个月后，栽培品种Vinhão的自生根葡萄插条在叶片中积累> 0.2％Si（DW），而在对照叶片中未检测到Si。实时PCR显示，VvNIP2; 1在根和绿色浆果中高表达。根癌农杆菌介导的烟草叶表皮细胞的瞬时转化证实VvNIP2; 1定位在质膜上。在卵母细胞中的转运实验表明，VvNIP2; 1介导了硅和砷的吸收，而通透性研究表明，酵母中表达的VvNIP2; 1无法转运水和甘油。向有色葡萄培养细胞（c。GamayFreáux）中添加硅对总酚和花青素含量，生长速度和VvNIP2; 1表达没有影响。长期实验应有助于确定一段时间内对硅的吸收程度以及葡萄是否可以从硅施肥中受益。