Abstract Metabolic syndrome (MS) has been related with alterations in expression levels of orphan G protein coupled receptors (GPCRs) such as GPR21 and GPR82, which could be involved in some of the elements that characterizes the metabolic syndrome. The aim of this work was to evaluate changes in GPR21 and GPR82 receptors expression in two models of metabolic syndrome: one genetic (Zucker rats), and the other based on a diet (70% fructose for 9 weeks). GPR21 and GPR82 gene expressions were evaluated in brain, heart, aorta, liver and kidney by RT-qPCR. Rats with a high fructose diet, as well as obese Zucker rats, showed initial stages of pancreatic damage and alterations in some biochemical parameters related to the model consistent with the classification of MS. GPR21 and GPR82 receptors expressed in all tissues. The expression of GPR21 decreased in heart, aorta and kidney, but in liver the expression was different: decreased in diet model and increased in genetic model. In contrast, GPR82 expression depended of tissue and metabolic syndrome model. The results highlight the possible role of GPR21 and GPR82 receptors in the development MS. We conclude that the expression of GPR21 and GPR82 in different tissues is related with MS and depend of the origin of the syndrome, so they could be a therapeutic target for that syndrome.

中文翻译：

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代谢综合征病因导致 GPR21 和 GPR82 基因表达的改变

摘要 代谢综合征 (MS) 与孤儿 G 蛋白偶联受体 (GPCR) 表达水平的改变有关，例如 GPR21 和 GPR82，这可能与代谢综合征的某些特征有关。这项工作的目的是评估两种代谢综合征模型中 GPR21 和 GPR82 受体表达的变化：一种是遗传性的（Zucker 大鼠），另一种是基于饮食（70% 果糖，持续 9 周）。通过 RT-qPCR 在脑、心脏、主动脉、肝脏和肾脏中评估 GPR21 和 GPR82 基因表达。高果糖饮食的大鼠以及肥胖的 Zucker 大鼠显示出胰腺损伤的初始阶段和与 MS 分类一致的模型相关的一些生化参数的改变。GPR21 和 GPR82 受体在所有组织中表达。GPR21在心脏、主动脉和肾脏中的表达降低，但在肝脏中表达不同：饮食模型中降低，遗传模型中增加。相反，GPR82 表达取决于组织和代谢综合征模型。结果突出了 GPR21 和 GPR82 受体在 MS 发展中的可能作用。我们得出结论，不同组织中 GPR21 和 GPR82 的表达与 MS 相关并取决于综合征的起源，因此它们可能是该综合征的治疗靶点。