Vibrio harveyi is one of the pathogens that threaten the shrimp farming industry. However, metabolic changes induced by V. harveyi infection in shrimp remain unknown. In this study, we first conducted high resolution-magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR)-based metabolomics studies on gill, hepatopancreas, and haemolymph of V. harveyi-infected white leg shrimp, Litopenaeus vannamei. Using multivariate statistical analysis, we observed a clear separation between the early (3 and 9 h post-injection (hpi)) and late phases (24, 72 and 144 hpi) of the infection in all tissues. Moreover, metabolic changes in response to V. harveyi infection were faster in the haemolymph in the early phase and significantly changed in the late phase of the infection in the gills. Extensive changes were observed in the hepatopancreas, with 24 hpi being the turning point of progression from early to late phase infection in the hepatopancreas. V. harveyi infection increased the energy demand in L. vannamei and the amino acid and carbohydrate metabolism pathways also exhibited significant changes depending on the tissue. Thus, each tissue displayed different metabolic changes, depending on the progress of the infection.

中文翻译：

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哈维氏弧菌感染显着改变了南美白对虾南美白对虾的氨基酸和碳水化合物代谢。

哈维弧菌是威胁虾类养殖业的病原体之一。然而，虾中哈维氏弧菌感染引起的代谢变化仍然未知。在这项研究中，我们首先进行了基于高分辨率魔角旋转（HR-MAS）核磁共振（NMR）的代谢组学研究，研究了感染哈维伊弧菌的白腿虾南美白对虾（Litopenaeus vannamei）的meta，肝胰腺和血淋巴。使用多变量统计分析，我们观察到所有组织中感染的早期（注射后3h和9h）和晚期（24、72和144 hpi）之间有明显的分离。此外，对哈维弧菌的代谢变化感染早期在血淋巴中感染较快，而在the感染后期则明显改变。在肝胰腺中观察到广泛的变化，其中24hpi是肝胰腺从早期到晚期感染发展的转折点。哈维氏弧菌感染增加了南美白对虾的能量需求，并且氨基酸和碳水化合物的代谢途径也根据组织表现出显着变化。因此，根据感染的进展，每个组织显示出不同的代谢变化。