Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.

活的单个酵母细胞表现出纳米级的特定细胞运动，其幅度与细胞的细胞活性成正比。我们使用经典光学显微镜表征了非附着单个酵母细胞的细胞纳米运动模式。短时间内细胞位移的分布与随机运动不同。这种纳米运动位移分布的范围和形状根据细胞的代谢状态而显着变化。对纳米运动频率模式的分析表明，单个活酵母细胞以 2 赫兹左右的相对较低频率振荡。该技术的简单性将为众多应用开辟道路，其中抗真菌药敏试验似乎是最简单的。