当前位置：
X-MOL 学术
›
J. Integr. Plant Biol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Glutamate synthase 1 is involved in iron-deficiency response and long-distance transportation in Arabidopsis.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-06-25 , DOI: 10.1111/jipb.12985 Man Cui 1, 2 , Mengjun Gu 1, 2 , Yaru Lu 1 , Yue Zhang 1 , Chunlin Chen 1 , Hong-Qing Ling 1, 2 , Huilan Wu 1
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-06-25 , DOI: 10.1111/jipb.12985 Man Cui 1, 2 , Mengjun Gu 1, 2 , Yaru Lu 1 , Yue Zhang 1 , Chunlin Chen 1 , Hong-Qing Ling 1, 2 , Huilan Wu 1
Affiliation
|
Iron is an essential microelement for plant growth. After uptake from the soil, iron is chelated by ligands and translocated from roots to shoots for subsequent utilization. However, the number of ligands involved in iron chelation is unclear. In this study, we identified and demonstrated that GLU1, which encodes a ferredoxin‐dependent glutamate synthase, was involved in iron homeostasis. First, the expression of GLU1 was strongly induced by iron deficiency condition. Second, lesion of GLU1 results in reduced transcription of many iron‐deficiency‐responsive genes in roots and shoots. The mutant plants revealed a decreased iron concentration in the shoots, and displayed severe leaf chlorosis under the condition of Fe limitation, compared to wild‐type. Third, the product of GLU1, glutamate, could chelate iron in vivo and promote iron transportation. Last, we also found that supplementation of glutamate in the medium can alleviate cadmium toxicity in plants. Overall, our results provide evidence that GLU1 is involved in iron homeostasis through affecting glutamate synthesis under iron deficiency conditions in Arabidopsis.
中文翻译：
谷氨酸合酶1参与拟南芥中的铁缺乏反应和长距离运输。
铁是植物生长必不可少的微量元素。从土壤中吸收后，铁被配体螯合，并从根部转移到芽，以备后用。然而，尚不清楚参与铁螯合的配体数目。在这项研究中，我们鉴定并证明了编码铁氧还蛋白依赖性谷氨酸合酶的GLU1参与了铁稳态。首先，铁缺乏条件强烈诱导了GLU1的表达。二，GLU1的病变导致根和茎中许多铁缺乏反应基因的转录减少。与野生型相比，该突变植物在芽中显示出铁浓度降低，并且在铁限制条件下表现出严重的叶绿化。第三，GLU1的产物谷氨酸可在体内螯合铁并促进铁的运输。最后，我们还发现在培养基中添加谷氨酸盐可以减轻植物对镉的毒性。总的来说，我们的结果提供了证据，表明在拟南芥中铁缺乏条件下，GLU1通过影响谷氨酸的合成而参与了铁稳态。
更新日期：2020-06-25
中文翻译：
谷氨酸合酶1参与拟南芥中的铁缺乏反应和长距离运输。
铁是植物生长必不可少的微量元素。从土壤中吸收后，铁被配体螯合，并从根部转移到芽，以备后用。然而，尚不清楚参与铁螯合的配体数目。在这项研究中，我们鉴定并证明了编码铁氧还蛋白依赖性谷氨酸合酶的GLU1参与了铁稳态。首先，铁缺乏条件强烈诱导了GLU1的表达。二，GLU1的病变导致根和茎中许多铁缺乏反应基因的转录减少。与野生型相比，该突变植物在芽中显示出铁浓度降低，并且在铁限制条件下表现出严重的叶绿化。第三，GLU1的产物谷氨酸可在体内螯合铁并促进铁的运输。最后，我们还发现在培养基中添加谷氨酸盐可以减轻植物对镉的毒性。总的来说，我们的结果提供了证据，表明在拟南芥中铁缺乏条件下，GLU1通过影响谷氨酸的合成而参与了铁稳态。
京公网安备 11010802027423号