• Abscisic acid (ABA) and jasmonic acid (JA) both inhibit seed germination, but their interactions during this process remain elusive. Here, we report the identification of a ‘SAPK10‐bZIP72‐AOC’ pathway, through which ABA promotes JA biosynthesis to synergistically inhibit rice seed germination.
• Using biochemical interaction and phosphorylation assays, we show that SAPK10 exhibits autophosphorylation activity on the 177^th serine, which enables it to phosphorylate bZIP72 majorly on 71^st serine. The SAPK10‐dependent phosphorylation enhances bZIP72 protein stability as well as the DNA‐binding ability to the G‐box cis‐element of AOC promoter, thereby elevating the AOC transcription and the endogenous concentration of JA.
• Blocking of JA biosynthesis significantly alleviated the ABA sensitivity on seed germination, suggesting that ABA‐imposed inhibition partially relied on the elevated concentration of JA.
• Our findings shed a novel insight into the molecular networks of ABA–JA synergistic interaction during rice seed germination.

中文翻译：

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脱落酸通过“SAPK10-bZIP72-AOC”途径促进茉莉酸生物合成，从而协同抑制水稻 (Oryza sativa) 种子的萌发。

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  脱落酸 (ABA) 和茉莉酸 (JA) 都会抑制种子发芽，但在此过程中它们的相互作用仍然难以捉摸。在这里，我们报告了“SAPK10-bZIP72- AOC ”途径的鉴定，ABA通过该途径促进JA生物合成，从而协同抑制水稻种子发芽。
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  使用生化相互作用和磷酸化测定，我们表明 SAPK10 在第 177^号丝氨酸上表现出自磷酸化活性，这使其能够主要在第 71^号丝氨酸上磷酸化 bZIP72。 SAPK10 依赖的磷酸化增强了 bZIP72 蛋白的稳定性以及AOC启动子 G-box顺式元件的 DNA 结合能力，从而提高了AOC转录和 JA 的内源浓度。
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  阻断 JA 生物合成可显着减轻 ABA 对种子萌发的敏感性，表明 ABA 施加的抑制部分依赖于 JA 浓度的升高。
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  我们的研究结果为水稻种子萌发过程中 ABA-JA 协同相互作用的分子网络提供了新的见解。