LIPS method for the detection of SARS-CoV-2 antibodies to spike and nucleocapsid proteins.,European Journal of Immunology

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LIPS method for the detection of SARS-CoV-2 antibodies to spike and nucleocapsid proteins.
European Journal of Immunology ( IF 4.5 ) Pub Date : 2020-06-25 , DOI: 10.1002/eji.202048715
Liis Haljasmägi ₁ , Anu Remm ₁ , Anna Pauliina Rumm ₁ , Ekaterina Krassohhina ₁ , Hanna Sein ₁ , Anu Tamm ₂ , Kai Kisand ₁ , Pärt Peterson ₁

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Profiling antibodies to SARS‐CoV‐2 can help to assess potential immune response after COVID‐19 disease. Luciferase IP system (LIPS) assay is a sensitive method for quantitative detection of antibodies to antigens in their native conformation. We here describe LIPS to detect antibody responses to SARS‐CoV‐2 spike (S) and nucleocapsid (N) proteins in COVID‐19 patients. The antibodies targeted both S and N fragments and gave a high assay sensitivity by identifying 26 out of 26 COVID‐19 patients with N antigen or with three protein fragments when combined into a single reaction. The assay correlated well with ELISA method and was specific to COVID‐19 as we saw no reactivity among uninfected healthy controls. Our results show that LIPS is a rapid and measurable method to screen antibody responses against SARS‐CoV‐2 antigens.

中文翻译：

LIPS 方法用于检测 SARS-CoV-2 刺突蛋白和核衣壳蛋白抗体。

分析 SARS-CoV-2 抗体有助于评估 COVID-19 疾病后潜在的免疫反应。荧光素酶 IP 系统 (LIPS) 测定是一种灵敏的方法，用于定量检测天然构象的抗原抗体。我们在这里描述了 LIPS，用于检测 COVID-19 患者对 SARS-CoV-2 刺突 (S) 和核衣壳 (N) 蛋白的抗体反应。这些抗体同时靶向 S 和 N 片段，并通过在单一反应中识别出 26 名 COVID-19 患者中的 26 名具有 N 抗原或具有三个蛋白质片段，提供了高检测灵敏度。该检测与 ELISA 方法相关性良好，并且对 COVID-19 具有特异性，因为我们在未感染的健康对照中没有发现任何反应。我们的结果表明，LIPS 是一种快速且可测量的方法，用于筛选针对 SARS-CoV-2 抗原的抗体反应。

更新日期：2020-08-04

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