Onion is one of the major vegetable crops in terms of production as well as consumption. In the current research, available onion genetic stock was evaluated to identify male‐sterile lines and produce high‐yielding F₁ hybrids for future breeding programmes. A mitochondrial DNA‐based marker was mapped and correlated with phenotypic traits to isolate male‐sterile plants. Based on the floral and pollen structure, nine putative male‐sterile lines were identified. On the other hand, for nuclear marker identification at Ms locus, two sets of primers were used, one for Ms dominant allele and another for sterile and maintainer plants. Results revealed that 70% of open pollinated varieties (OPVs) possess plants with sterile cytoplasm coupled with genetic sterility at Ms locus, called sterile “A” line. Approximately 20% of plants in some genotypes were identified with normal (N) cytoplasm having recessive fertility gene at Ms locus, called maintainer “B” line. Based on the present findings, “A”, “B” and “R” (restorer line), future F₁ hybrid seed production systems in onion is discussed.

中文翻译：

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从洋葱的高级育种程序中鉴定和鉴定来自各种基因型的重要无菌和保持系

就生产和消费而言，洋葱是主要的蔬菜作物之一。在当前的研究中，对可用的洋葱遗传储备进行了评估，以鉴定雄性不育品系并为未来的育种计划生产高产的F ₁杂种。绘制了基于线粒体DNA的标记，并将其与表型性状相关联以分离雄性不育植物。根据花粉和花粉结构，鉴定出九种假定的雄性不育系。另一方面，为了在Ms位点鉴定核标记，使用了两组引物，一组用于Ms优势等位基因，另一组用于不育和维持植物。结果表明，70％的开放授粉变种（OPV）拥有带有不育细胞质且遗传不育的植物。位点女士，称为无菌“ A”线。在某些基因型中，大约有20％的植物被鉴定为在Ms位点具有隐性育性基因的正常（N）细胞质，称为维持系“ B”系。基于目前的发现，“ A”，“ B”和“ R”（恢复系），讨论了未来的洋葱F ₁杂交种子生产系统。