Crop improvement in sorghum has progressed extensively availing the variation available in the primary gene pool of S. bicolor. Crossing further, the yield plateau can be accomplished by bringing in the genes from tertiary gene pool into the cultivated gene pool. Earlier efforts for wide hybridization in sorghum with tertiary gene pool were not successful due to inhibition of wild pollen germination on the stigma of cultivated species. In the current study, elite lines of cultivated Sorghum species were pollinated with wild species using different floral treatments. The results showed that spray with the synthetic auxin, 2,4‐dichlorophenoxyacetic acid, has promoted the seed set. Overall, 76% crosses showed seed set, but many seeds were underdeveloped and deformed. F₁ progeny showed traits of both cultivated and wild parents. Hybrids were confirmed through PCR analysis. Many (80%) F₁ plants were sterile, and 20% of F₁s were advanced to F₂ generation. Wide variation was observed in F₂ progeny, and the phenotype selections were made and are being evaluated in the field.

中文翻译：

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克服高粱种间障碍和衍生物的分子表征

高粱的作物改良取得了很大进展，充分利用了双色链球菌一级基因库中的变异。进一步交叉，可以通过将三级基因库中的基因引入培养的基因库中来实现产量的稳定。高粱与三级基因库广泛杂交的早期努力未成功，原因是抑制了野生花粉萌发对栽培种的柱头。在当前的研究中，使用不同的花卉处理方法将野生高粱授粉的栽培高粱品种的优良品系。结果表明，喷洒合成生长素2,4-二氯苯氧基乙酸可以促进种子结实。总体而言，76％的杂交显示出结实，但许多种子发育不全且变形。˚F ₁后代表现出栽培父母和野生父母的特征。通过PCR分析确认杂种。许多（80％）F ₁植物是不育的，并且20％的F ₁发育为F ₂代。在F ₂子代中观察到广泛的变异，并且进行了表型选择并正在现场进行评估。