This 14‐color flow cytometry panel was designed to identify newly described subpopulations within human group 2 innate lymphoid cells (ILC2s) and other ILC subsets. This panel also allowed to identify recently reported subpopulations of peripheral blood CRTH2⁻ c‐Kit⁺ ILCs. We validated this panel mostly in human peripheral blood but also confirmed that the same panel and gating strategy works well in human tonsillar cells. The panel contains a few markers indicating the activation status of ILCs. In addition, phycoerythrin (PE) channel is available for the markers of interest in each study. In the validation studies described here, PE channel was used to test the expression of some markers. These features make this panel applicable for immunophenotyping of ILCs in various disease states. © 2020 International Society for Advancement of Cytometry

中文翻译：

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OMIP-066：鉴定外周血中人类 2 组先天淋巴细胞的新亚群。

该 14 色流式细胞术面板旨在识别人类第 2 组先天淋巴细胞 (ILC2) 和其他 ILC 亚群中新描述的亚群。该小组还允许识别最近报告的外周血 CRTH2 ^- c-Kit ⁺亚群国际劳工大会。我们主要在人类外周血中验证了该面板，但也证实了相同的面板和门控策略在人类扁桃体细胞中运作良好。该面板包含一些指示 ILC 激活状态的标记。此外，藻红蛋白 (PE) 通道可用于每项研究中的感兴趣标记。在这里描述的验证研究中，PE 通道用于测试一些标记的表达。这些特征使该面板适用于各种疾病状态下 ILC 的免疫表型分析。© 2020 国际细胞计量学促进会