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THCH as electron donor in controlled-release system for procalcitonin analysis based on Bi2Sn2O7 photoanode
Sensors and Actuators B: Chemical ( IF 8.0 ) Pub Date : 2020-06-25 , DOI: 10.1016/j.snb.2020.128509
Dongquan Leng , Jingshuai Li , Rui Xu , Lei Liu , Xuejing Liu , Dawei Fan , Huan Wang , Qin Wei , Huangxian Ju

A split-type photoelectrochemical (PEC) sensor for procalcitonin (PCT) detection was successfully prepared. This split-type sensor adopted controlled-release strategy, which separated the two processes of antigen-antibody specific recognition and photoelectric conversion, so that the two processes can be performed independently. In addition, CdS sensitized Bi2Sn2O7 is combined for the first time to form a heterojunction as the matrix to provide the basic PEC response, which could effectively absorb visible-light, and reduced electron-hole pair recombination, ensured the stability of the sensor. Distinctly important, the skillfully process was performed in a 96-well plate where the specific reaction was completed proceeded between antigen and antibody. For improving sensitivity of the sensor, acetylcholinesterase (ACHE) connected to SiO2 nanospheres (SiO2 NSs) modified by second antibody (Ab2-ACHE@SiO2 NSs) was designed, the thiocholine (THCH) can be released when acetylthiocholine iodide (ATCH) exists, and the THCH as an electron donor could enhance PEC response. The proposed PEC immunoassay with high-performance photosensitive materials, and the smart electron donor released strategy have a wide detection liner range from 0.0005 to 100 ng/mL with a low detection limit of 0.17 pg/mL, also provide great clinical diagnostic potential for other target proteins.



中文翻译:

THCH作为基于Bi 2 Sn 2 O 7光阳极的降钙素原分析的控释系统中的电子供体

成功制备了用于降钙素原(PCT)检测的分体式光电化学(PEC)传感器。这种分体式传感器采用控释策略,将抗原-抗体特异性识别和光电转换两个过程分开,因此这两个过程可以独立执行。此外,CdS敏化的Bi 2 Sn 2 O 7第一次结合,形成异质结作为基质,提供基本的PEC响应,可以有效吸收可见光,并减少电子-空穴对的重组,确保了传感器的稳定性。至关重要的是,熟练的过程是在96孔板中进行的,其中特异性反应在抗原和抗体之间完成。为了提高传感器的灵敏度,将乙酰胆碱酯酶(ACHE)连接到由第二抗体(Ab 2 -ACHE @ SiO 2)修饰的SiO 2纳米球(SiO 2 NSs)设计了NSs),当存在碘代乙酰胆碱(ATCH)时可以释放出硫代胆碱(THCH),并且THCH作为电子供体可以增强PEC反应。拟议的具有高性能光敏材料的PEC免疫测定和智能电子供体释放策略具有从0.0005到100 ng / mL的宽检测衬范围和0.17 pg / mL的低检测限,也为其他检测提供了巨大的临床诊断潜力靶蛋白。

更新日期:2020-06-25
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