A split-type photoelectrochemical (PEC) sensor for procalcitonin (PCT) detection was successfully prepared. This split-type sensor adopted controlled-release strategy, which separated the two processes of antigen-antibody specific recognition and photoelectric conversion, so that the two processes can be performed independently. In addition, CdS sensitized Bi₂Sn₂O₇ is combined for the first time to form a heterojunction as the matrix to provide the basic PEC response, which could effectively absorb visible-light, and reduced electron-hole pair recombination, ensured the stability of the sensor. Distinctly important, the skillfully process was performed in a 96-well plate where the specific reaction was completed proceeded between antigen and antibody. For improving sensitivity of the sensor, acetylcholinesterase (ACHE) connected to SiO₂ nanospheres (SiO₂ NSs) modified by second antibody (Ab₂-ACHE@SiO₂ NSs) was designed, the thiocholine (THCH) can be released when acetylthiocholine iodide (ATCH) exists, and the THCH as an electron donor could enhance PEC response. The proposed PEC immunoassay with high-performance photosensitive materials, and the smart electron donor released strategy have a wide detection liner range from 0.0005 to 100 ng/mL with a low detection limit of 0.17 pg/mL, also provide great clinical diagnostic potential for other target proteins.

成功制备了用于降钙素原（PCT）检测的分体式光电化学（PEC）传感器。这种分体式传感器采用控释策略，将抗原-抗体特异性识别和光电转换两个过程分开，因此这两个过程可以独立执行。此外，CdS敏化的Bi ₂ Sn ₂ O ₇第一次结合，形成异质结作为基质，提供基本的PEC响应，可以有效吸收可见光，并减少电子-空穴对的重组，确保了传感器的稳定性。至关重要的是，熟练的过程是在96孔板中进行的，其中特异性反应在抗原和抗体之间完成。为了提高传感器的灵敏度，将乙酰胆碱酯酶（ACHE）连接到由第二抗体（Ab ₂ -ACHE @ SiO ₂）修饰的SiO ₂纳米球（SiO ₂ NSs）设计了NSs），当存在碘代乙酰胆碱（ATCH）时可以释放出硫代胆碱（THCH），并且THCH作为电子供体可以增强PEC反应。拟议的具有高性能光敏材料的PEC免疫测定和智能电子供体释放策略具有从0.0005到100 ng / mL的宽检测衬范围和0.17 pg / mL的低检测限，也为其他检测提供了巨大的临床诊断潜力靶蛋白。