Cancer is still remain as a global burden with the 18.1 million and 9.6 million new cases and mortlities, respectively estimated globally. Leukemia may arise at all ages varied from the infants to elders. In this exploration, we planned to evaluate the antiproliferative effect of D-pinitol on human leukemia MOLT-4 cells. Anticancer potential of D-pinitol was examined using MTT assay. Reactive oxygen species (ROS) generation was studied by fluorescence microscopic method using DCFH-DA staining. Apoptotic morphological alterations were determined by dual staining (acridine orange and ethidium bromide). Western blot and ELISA methods were employed to study apoptotic protein expression. D-pinitol treatment significantly induced cytotoxicity in human leukemia MOLT-4 cells. We observed that D-pinitol induces the generation of ROS in MOLT-4 cells. Further, we noticed that D-pinitol significantly induced apoptosis in a dosage dependent manner. Moreover, western blot and ELISA based analysis revealed that D-pinitol elevated the Bax, Caspase-3, Caspase-9 and attenuated the Bcl-2 expression in leukemic cancer cell. Our findings suggest that D-pinitol treatment induces the apoptosis in human leukemic cells by generating intracellular ROS and modulating apoptotic protein expression.

癌症仍然是全球负担，据估计全球分别有1810万和960万新病例和死亡。白血病可能出现在各个年龄段，从婴儿到老年人。在这项探索中，我们计划评估D-松醇对人白血病MOLT-4细胞的抗增殖作用。使用MTT测定法检查了D-松醇的抗癌潜力。使用DCFH-DA染色通过荧光显微镜方法研究了活性氧（ROS）的产生。通过双重染色（ac啶橙和溴化乙锭）确定细胞凋亡的形态学改变。采用Western blot和ELISA方法研究凋亡蛋白的表达。D-松醇治疗显着诱导人白血病MOLT-4细胞的细胞毒性。我们观察到D-松醇可诱导MOLT-4细胞中ROS的产生。此外，我们注意到D-松露醇以剂量依赖性方式显着诱导凋亡。此外，基于蛋白质印迹和ELISA的分析表明，D-松醇在白血病细胞中升高了Bax，Caspase-3，Caspase-9并减弱了Bcl-2的表达。我们的发现表明，D-松醇治疗可通过产生细胞内ROS并调节凋亡蛋白表达来诱导人白血病细胞凋亡。