We propose a procedure for the growth of thin films of organic and bio-molecules, which is based on MAPLE (Matrix Assisted Pulsed Laser Evaporation) and inspired by the laser cleaning technique. This procedure is MAPLE-like, meaning that the “evaporation”, or better the ejection of the material to deposit, is not assisted by the matrix, but rather by the target-holder. The target-holder is basalt, a magmatic rock which is able to absorb the laser radiation used (1064 nm) thanks to its high content in iron oxides. A thin veil of solution that contains the molecule of interest is placed into the basalt cup, frozen and irradiated. The laser radiation reaches the bottom of the cup, where it is absorbed. The consequent increase in temperature generates the phase explosion of the overlying frozen matrix. This procedure can be used to obtain thin films from transparent matrices. Here we deposited the enzyme β-glucosidase highly preserving its native conformation. The laser power used was under the ablation threshold of the basalt, so that only few fragments of the rock reached the substrate.

我们提出了一种基于MAPLE（基质辅助脉冲激光蒸发）并受激光清洁技术启发的有机和生物分子薄膜生长程序。该过程类似于MAPLE，这意味着“蒸发”或更好地喷射出要沉积的材料，不是由基质而是由靶材保持器辅助的。目标持有者是玄武岩，它是一种岩浆岩石，由于其在氧化铁中含量高，因此能够吸收所用的激光辐射（1064 nm）。将含有目标分子的薄薄溶液溶液放入玄武岩杯中，冷冻并照射。激光辐射到达杯子的底部并被吸收。因此，温度升高会导致上面的冷冻基质发生相爆炸。此过程可用于从透明矩阵中获取薄膜。在这里，我们沉积了高度保留其天然构象的酶β-葡萄糖苷酶。所使用的激光功率低于玄武岩的烧蚀阈值，因此只有很少的岩石碎片到达基底。