Abstract The native fluorescence of both rosuvastatin and amlodipine was investigated to carry out a new facile method for their simultaneous determination based on synchronous fluorimetric technique. It was found that addition of Tween-80 in a concentration above its critical micelle concentration causes significant increase in the sensitivity of the measurements for the two studied drugs. The developed method measured the first derivative synchronous amplitude (1D) for rosuvastatin at the zero-crossing point of amlodipine and vice versa over the linear ranges of (1.0–10.0 µg/mL) and (0.2–5.0 µg/mL) for rosuvastatin and amlodipine, respectively. All the validation criteria were assessed according to ICH guidelines. The method was further continued to the determination of the two drugs in pure forms, synthetic mixtures, single and combined Tablets with good percentage recoveries that ranged from about (98.0–102.0%). A comparison between the results of the suggested spectrofluorimetric method and previous reports for determination of each of the studied analytes has been carried out and statistical analysis revealed good agreement.

中文翻译：

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瑞舒伐他汀和氨氯地平固定剂量复方片同时测定中胶束增强的研究

摘要 研究了瑞舒伐他汀和氨氯地平的天然荧光，建立了一种基于同步荧光技术同时测定它们的简便方法。发现添加浓度高于其临界胶束浓度的 Tween-80 会显着提高两种研究药物的测量灵敏度。开发的方法在氨氯地平的零交叉点处测量了瑞舒伐他汀的一阶导数同步幅度 (1D)，反之亦然，在瑞舒伐他汀的线性范围 (1.0–10.0 µg/mL) 和 (0.2–5.0 µg/mL) 内，氨氯地平，分别。所有验证标准均根据 ICH 指南进行评估。该方法进一步用于测定纯形式、合成混合物、单片和组合片具有良好的回收率，范围为约 (98.0-102.0%)。已对建议的荧光分光光度法的结果与之前用于测定每种研究分析物的报告进行了比较，统计分析显示出良好的一致性。